F530XL
Thermo Scientific™ Phusion™ High-Fidelity DNA Polymerases (2 U/μL)
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | F530XL-Each-of-1 | In Stock | ₹ 1,90,798.80 |
F530XL - Each of 1
In Stock
Quantity
1
Base Price: ₹ 1,90,798.80
GST (18%): ₹ 34,343.784
Total Price: ₹ 2,25,142.584
Content And Storage
• Phusion DNA Polymerase (2 U/μL)• 5X Phusion HF & GC Buffers• DMSO• 50 mM MgCl2solutionPhusion HF Buffer and Phusion GC Buffers each provide 1.5 mM MgCl2in the final 1X concentration.Store at -20°C.
Polymerase
Phusion High Fidelity DNA Polymerase
Product Type
High-Fidelity DNA Polymerase
Shipping Condition
Dry Ice
Concentration
2 U/μL
Hot Start
No
Overhang
Blunt
Size (Final Product)
20 kb or less
GC-Rich PCR Performance
High
Reaction Speed
Fast
Quantity
2000 U
For Use With (Application)
Standard PCR, High-fidelity PCR
Fidelity (vs. Taq)
52X
No. of Reactions
2000 Reactions
Reaction Format
Standalone
Color
Colorless
Related Products
Description
- Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR
- Featuring an error rate 50-fold lower than that of Taq and 6-fold lower than that of Pfu , Phusion High-Fidelity DNA Polymerase is an excellent choice for cloning and other applications requiring high fidelity
- Phusion DNA polymerases offer robust performance with short protocol times, even in the presence of PCR inhibitors, and generate higher yields with lower enzyme amounts than other DNA polymerases
- Features of Phusion™ High-Fidelity DNA Polymerase include: High fidelity (52X Taq ) Fast PCR due to short extension times (15–30 s/kb) Robust performance, minimal optimization needed High yields of PCR products with minimal enzyme amounts Available in Green buffer format for direct loading of PCR products on gels (F-534S or F-534L) Applications High-fidelity PCR Cloning Template generation for sequencing Amplification of difficult (GC-rich) templates Long-range PCR (up to 20 kb) Mutagenesis High throughput PCR Microarray Note: Annealing rules for Phusion DNA polymerases are different from many common DNA polymerases (such as Taq DNA polymerases)
- For optimal results, use our convenient Tm calculator.
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Content And Storage: • Phusion DNA Polymerase (2 U/μL)• 5X Phusion HF & GC Buffers• DMSO• 50 mM MgCl2solutionPhusion HF Buffer and Phusion GC Buffers each provide 1.5 mM MgCl2in the final 1X concentration.Store at -20°C.
Polymerase: Phusion High Fidelity DNA Polymerase
Product Type: High-Fidelity DNA Polymerase
Shipping Condition: Dry Ice
Concentration: 2 U/μL
Hot Start: No
Overhang: Blunt
Size (Final Product): 20 kb or less
GC-Rich PCR Performance: High
Reaction Speed: Fast
Quantity: 2000 U
For Use With (Application): Standard PCR, High-fidelity PCR
Fidelity (vs. Taq): 52X
No. of Reactions: 2000 Reactions
Reaction Format: Standalone
Color: Colorless
Content And Storage:
• Phusion DNA Polymerase (2 U/μL)• 5X Phusion HF & GC Buffers• DMSO• 50 mM MgCl2solutionPhusion HF Buffer and Phusion GC Buffers each provide 1.5 mM MgCl2in the final 1X concentration.Store at -20°C.
Polymerase:
Phusion High Fidelity DNA Polymerase
Product Type:
High-Fidelity DNA Polymerase
Shipping Condition:
Dry Ice
Concentration:
2 U/μL
Hot Start:
No
Overhang:
Blunt
Size (Final Product):
20 kb or less
GC-Rich PCR Performance:
High
Reaction Speed:
Fast
Quantity:
2000 U
For Use With (Application):
Standard PCR, High-fidelity PCR
Fidelity (vs. Taq):
52X
No. of Reactions:
2000 Reactions
Reaction Format:
Standalone
Color:
Colorless
Content And Storage: __
Polymerase: __
Product Type: __
Shipping Condition: __
Concentration: __
Hot Start: __
Overhang: __
Size (Final Product): __
GC-Rich PCR Performance: __
Reaction Speed: __
Quantity: __
For Use With (Application): __
Fidelity (vs. Taq): __
No. of Reactions: __
Reaction Format: __
Color: __
Content And Storage:
__
Polymerase:
__
Product Type:
__
Shipping Condition:
__
Concentration:
__
Hot Start:
__
Overhang:
__
Size (Final Product):
__
GC-Rich PCR Performance:
__
Reaction Speed:
__
Quantity:
__
For Use With (Application):
__
Fidelity (vs. Taq):
__
No. of Reactions:
__
Reaction Format:
__
Color:
__
Content And Storage: • 2X Phusion Master Mix with HF Buffer (10 x 1.25 mL)• 100% DMSO (2 x 500 μL)Store at –20°C.
Polymerase: Phusion High-Fidelity DNA Polymerase
Product Type: High-Fidelity PCR Master Mix
Shipping Condition: Dry Ice
Concentration: 2X
Hot Start: No
Overhang: Blunt
Size (Final Product): 20 kb or less
GC-Rich PCR Performance: Medium
Reaction Speed: Fast
Quantity: __
For Use With (Application): Standard PCR, High-fidelity PCR
Fidelity (vs. Taq): 52X
No. of Reactions: 500 Reactions
Reaction Format: SuperMix or Master Mix
Color: __
Content And Storage:
• 2X Phusion Master Mix with HF Buffer (10 x 1.25 mL)• 100% DMSO (2 x 500 μL)Store at –20°C.
Polymerase:
Phusion High-Fidelity DNA Polymerase
Product Type:
High-Fidelity PCR Master Mix
Shipping Condition:
Dry Ice
Concentration:
2X
Hot Start:
No
Overhang:
Blunt
Size (Final Product):
20 kb or less
GC-Rich PCR Performance:
Medium
Reaction Speed:
Fast
Quantity:
__
For Use With (Application):
Standard PCR, High-fidelity PCR
Fidelity (vs. Taq):
52X
No. of Reactions:
500 Reactions
Reaction Format:
SuperMix or Master Mix
Color:
__
Content And Storage: • 2X Phusion Master Mix with HF Buffer (2 x 1.25 mL)• 100% DMSO (1 x 500 μL)Store at –20°C.
Polymerase: Phusion High-Fidelity DNA Polymerase
Product Type: High-Fidelity PCR Master Mix
Shipping Condition: Dry Ice
Concentration: 2X
Hot Start: No
Overhang: Blunt
Size (Final Product): 20 kb or less
GC-Rich PCR Performance: Medium
Reaction Speed: Fast
Quantity: __
For Use With (Application): Standard PCR, High-fidelity PCR
Fidelity (vs. Taq): 52X
No. of Reactions: 100 Reactions
Reaction Format: SuperMix or Master Mix
Color: __
Content And Storage:
• 2X Phusion Master Mix with HF Buffer (2 x 1.25 mL)• 100% DMSO (1 x 500 μL)Store at –20°C.
Polymerase:
Phusion High-Fidelity DNA Polymerase
Product Type:
High-Fidelity PCR Master Mix
Shipping Condition:
Dry Ice
Concentration:
2X
Hot Start:
No
Overhang:
Blunt
Size (Final Product):
20 kb or less
GC-Rich PCR Performance:
Medium
Reaction Speed:
Fast
Quantity:
__
For Use With (Application):
Standard PCR, High-fidelity PCR
Fidelity (vs. Taq):
52X
No. of Reactions:
100 Reactions
Reaction Format:
SuperMix or Master Mix
Color:
__