F548S
Thermo Scientific™ Phusion Flash High-Fidelity PCR Master Mix
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | F548S-Each-of-1 | In Stock | ₹ 11,445.40 |
F548S - Each of 1
In Stock
Quantity
1
Base Price: ₹ 11,445.40
GST (18%): ₹ 2,060.172
Total Price: ₹ 13,505.572
Content And Storage
Phusion Flash High-Fidelity PCR Master Mix (1 mL)Store at -20°C.
Polymerase
Phusion Flash II DNA Polymerase
Product Type
High-Fidelity PCR Master Mix
For Use With (Application)
Hot-start PCR, High-fidelity PCR
Fidelity (vs. Taq)
25X
No. of Reactions
100 Reactions
Reaction Format
SuperMix or Master Mix
Color
Colorless
GC-Rich PCR Performance
High
Reaction Speed
Fast
Shipping Condition
Dry Ice
Concentration
2X
Hot Start
Built-In Hot Start
Overhang
Blunt
Size (Final Product)
20 kb or less
Related Products
Description
- Thermo Scientific Phusion Flash PCR Master Mix was developed to save valuable laboratory time
- Ready-to-use 2X master mix preserves the fidelity and the yield in reactions when using very short PCR protocols
- The unique master mix composition permits usage of short cycling protocols with both low and high complexity DNA templates (15 seconds per kilobase or less)
- The master mix utilizes Phusion Flash II DNA Polymerase, a modified proofreading DNA polymerase derived from Phusion Hot Start II High-Fidelity DNA Polymerase
- Features Extreme speed with extension times of 15 s/kb or less Hot start modification allows 'zero-time reactivation' Accurate proofreading DNA polymerase with a fidelity of 25X Taq High yields in reduced cycling time Minimized pipetting steps Applications Fast PCR High-fidelity PCR Difficult (GC-rich) templates Template generation for sequencing Multiplex PCR Long-range PCR Cloning & mutagenesis Microarray Notes Annealing rules for Phusion DNA Polymerases are different from many common DNA polymerases (such as Taq DNA polymerases).
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Content And Storage: Phusion Flash High-Fidelity PCR Master Mix (1 mL)Store at -20°C.
Polymerase: Phusion Flash II DNA Polymerase
Product Type: High-Fidelity PCR Master Mix
For Use With (Application): Hot-start PCR, High-fidelity PCR
Fidelity (vs. Taq): 25X
No. of Reactions: 100 Reactions
Reaction Format: SuperMix or Master Mix
Color: Colorless
GC-Rich PCR Performance: High
Reaction Speed: Fast
Shipping Condition: Dry Ice
Concentration: 2X
Hot Start: Built-In Hot Start
Overhang: Blunt
Size (Final Product): 20 kb or less
Content And Storage:
Phusion Flash High-Fidelity PCR Master Mix (1 mL)Store at -20°C.
Polymerase:
Phusion Flash II DNA Polymerase
Product Type:
High-Fidelity PCR Master Mix
For Use With (Application):
Hot-start PCR, High-fidelity PCR
Fidelity (vs. Taq):
25X
No. of Reactions:
100 Reactions
Reaction Format:
SuperMix or Master Mix
Color:
Colorless
GC-Rich PCR Performance:
High
Reaction Speed:
Fast
Shipping Condition:
Dry Ice
Concentration:
2X
Hot Start:
Built-In Hot Start
Overhang:
Blunt
Size (Final Product):
20 kb or less
Content And Storage: • Phusion Hot Start II High-Fidelity DNA Polymerase (2 U/μL)• 5X Phusion HF & GC Buffers• DMSO• 50 mM MgCl2solutionPhusion HF Buffer and Phusion GC Buffers each provide 1.5 mM MgCl2in the final 1X concentration.Store at -20°C.
Polymerase: Phusion Hot Start II DNA Polymerase
Product Type: Hot Start DNA Polymerase
For Use With (Application): Hot-start PCR, High-fidelity PCR
Fidelity (vs. Taq): 52X
No. of Reactions: 500 Reactions
Reaction Format: Standalone
Color: Colorless
GC-Rich PCR Performance: High
Reaction Speed: Fast
Shipping Condition: Dry Ice
Concentration: 2 U/μL
Hot Start: Built-In Hot Start
Overhang: Blunt
Size (Final Product): 20 kb or less
Content And Storage:
• Phusion Hot Start II High-Fidelity DNA Polymerase (2 U/μL)• 5X Phusion HF & GC Buffers• DMSO• 50 mM MgCl2solutionPhusion HF Buffer and Phusion GC Buffers each provide 1.5 mM MgCl2in the final 1X concentration.Store at -20°C.
Polymerase:
Phusion Hot Start II DNA Polymerase
Product Type:
Hot Start DNA Polymerase
For Use With (Application):
Hot-start PCR, High-fidelity PCR
Fidelity (vs. Taq):
52X
No. of Reactions:
500 Reactions
Reaction Format:
Standalone
Color:
Colorless
GC-Rich PCR Performance:
High
Reaction Speed:
Fast
Shipping Condition:
Dry Ice
Concentration:
2 U/μL
Hot Start:
Built-In Hot Start
Overhang:
Blunt
Size (Final Product):
20 kb or less
Content And Storage: • Phusion Hot Start II High-Fidelity DNA Polymerase (4 x 500 U at 2 U/μL)• 10 mM dNTP Mix (2 x 1 mL)• 5X Phusion HF & GC Buffers• DMSO• 50 mM MgCl2solutionStore at –5°C to –30°C.
Polymerase: Phusion Hot Start II DNA Polymerase
Product Type: Hot Start DNA Polymerase and dNTP Mix
For Use With (Application): Hot-start PCR, High-fidelity PCR
Fidelity (vs. Taq): 52X
No. of Reactions: 2000 Reactions
Reaction Format: Separate Components
Color: __
GC-Rich PCR Performance: High
Reaction Speed: Fast
Shipping Condition: Dry Ice
Concentration: 2 U/μL
Hot Start: Built-In Hot Start
Overhang: Blunt
Size (Final Product): 20 kb or less
Content And Storage:
• Phusion Hot Start II High-Fidelity DNA Polymerase (4 x 500 U at 2 U/μL)• 10 mM dNTP Mix (2 x 1 mL)• 5X Phusion HF & GC Buffers• DMSO• 50 mM MgCl2solutionStore at –5°C to –30°C.
Polymerase:
Phusion Hot Start II DNA Polymerase
Product Type:
Hot Start DNA Polymerase and dNTP Mix
For Use With (Application):
Hot-start PCR, High-fidelity PCR
Fidelity (vs. Taq):
52X
No. of Reactions:
2000 Reactions
Reaction Format:
Separate Components
Color:
__
GC-Rich PCR Performance:
High
Reaction Speed:
Fast
Shipping Condition:
Dry Ice
Concentration:
2 U/μL
Hot Start:
Built-In Hot Start
Overhang:
Blunt
Size (Final Product):
20 kb or less
Content And Storage: • Phusion Hot Start II High-Fidelity DNA Polymerase (2 U/μL)• 5X Phusion HF & GC Buffers• DMSO• 50 mM MgCl2solutionPhusion HF Buffer and Phusion GC Buffers each provide 1.5 mM MgCl2in the final 1X concentration.Store at -20°C.
Polymerase: Phusion Hot Start II DNA Polymerase
Product Type: Hot Start DNA Polymerase
For Use With (Application): Hot-start PCR, High-fidelity PCR
Fidelity (vs. Taq): 52X
No. of Reactions: 100 Reactions
Reaction Format: Standalone
Color: Colorless
GC-Rich PCR Performance: High
Reaction Speed: Fast
Shipping Condition: Dry Ice
Concentration: 2 U/μL
Hot Start: Built-In Hot Start
Overhang: Blunt
Size (Final Product): 20 kb or less
Content And Storage:
• Phusion Hot Start II High-Fidelity DNA Polymerase (2 U/μL)• 5X Phusion HF & GC Buffers• DMSO• 50 mM MgCl2solutionPhusion HF Buffer and Phusion GC Buffers each provide 1.5 mM MgCl2in the final 1X concentration.Store at -20°C.
Polymerase:
Phusion Hot Start II DNA Polymerase
Product Type:
Hot Start DNA Polymerase
For Use With (Application):
Hot-start PCR, High-fidelity PCR
Fidelity (vs. Taq):
52X
No. of Reactions:
100 Reactions
Reaction Format:
Standalone
Color:
Colorless
GC-Rich PCR Performance:
High
Reaction Speed:
Fast
Shipping Condition:
Dry Ice
Concentration:
2 U/μL
Hot Start:
Built-In Hot Start
Overhang:
Blunt
Size (Final Product):
20 kb or less