FEREP0282
Thermo Scientific™ Taq DNA Polymerase, native (5 U/μL)
Manufacturer: Fischer Scientific
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | FEREP0282-Each-of-1 | In Stock | ₹ 24,726.84 |
FEREP0282 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 24,726.84
GST (18%): ₹ 4,450.831
Total Price: ₹ 29,177.671
Content And Storage
•TaqDNA Polymerase, native (5 U/μL), 100 μL• 10XTaqBuffer with KCl, 2 x 1.25 mL• 10XTaqBuffer with (NH4)2SO4, 2 x 1.25 mL• 25 mM MgCl2, 2 x 1.25 mLStore at -20°C.
GC-Rich PCR Performance
Low
Reaction Speed
Standard
Quantity
500 Units
Concentration
5 U/μL
Hot Start
No
Reaction Format
Separate Components
Volume
2 mL
Format
Without BSA
Polymerase
TaqDNA Polymerase
Product Type
DNA Polymerase
For Use With (Application)
Standard PCR
Fidelity (vs. Taq)
1X
Overhang
3'-A
Starting Material
DNA
Related Products
Description
- Thermo Scientific Taq DNA Polymerase, native, is a highly thermostable DNA polymerase of the thermophilic bacterium Thermus aquaticus
- The enzyme catalyzes 5' to 3' synthesis of DNA, has no detectable 3' to 5' exonuclease (proofreading) activity, and possesses low 5' to 3' exonuclease activity
- In addition, the polymerase exhibits deoxynucleotidyl transferase activity, which frequently results in the addition of extra adenines at the 3'-end of PCR products
- Native Taq DNA Polymerase is preferred for amplification of bacterial DNA sequences homologous to those found in E
- coli
- Available with or without the stabilizing agent BSA, Taq DNA Polymerase, native, with BSA, is often considered the best choice when amplifying DNA samples of lower purity, e.g., genomic DNA from mouse tail
- Both native Taq DNA polymerases are supplied with two buffers: 10X Taq Buffer with KCl and 10X Taq Buffer with (NH 4 ) 2 SO 4
- The latter allows for PCR at a wide range of magnesium concentrations and decreases unspecific priming Features Thermostable with half-life more than 40 min at 95°C Generates PCR products with 3'-dA overhangs Incorporates modified nucleotides (e.g., biotin-, digoxigenin-, fluorescent-labeled nucleotides) Applications Routine PCR amplification of DNA fragments up to 5 kb DNA labeling High throughput PCR Notes The error rate of Taq DNA Polymerase in PCR is 2.2 x 10 -5 errors per nt per cycle
- Accordingly, the accuracy of PCR is 4.5 x 10 4
- Accuracy is an inverse of the error rate and shows an average number of correct nucleotides incorporated before an error occurs
- The 10X Taq Buffer without Detergent is recommended for microarray experiments.
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Content And Storage: •TaqDNA Polymerase, native (5 U/μL), 100 μL• 10XTaqBuffer with KCl, 2 x 1.25 mL• 10XTaqBuffer with (NH4)2SO4, 2 x 1.25 mL• 25 mM MgCl2, 2 x 1.25 mLStore at -20°C.
GC-Rich PCR Performance: Low
Reaction Speed: Standard
Quantity: 500 Units
Concentration: 5 U/μL
Hot Start: No
Reaction Format: Separate Components
Volume: 2 mL
Format: Without BSA
Polymerase: TaqDNA Polymerase
Product Type: DNA Polymerase
For Use With (Application): Standard PCR
Fidelity (vs. Taq): 1X
Overhang: 3'-A
Starting Material: DNA
Content And Storage:
•TaqDNA Polymerase, native (5 U/μL), 100 μL• 10XTaqBuffer with KCl, 2 x 1.25 mL• 10XTaqBuffer with (NH4)2SO4, 2 x 1.25 mL• 25 mM MgCl2, 2 x 1.25 mLStore at -20°C.
GC-Rich PCR Performance:
Low
Reaction Speed:
Standard
Quantity:
500 Units
Concentration:
5 U/μL
Hot Start:
No
Reaction Format:
Separate Components
Volume:
2 mL
Format:
Without BSA
Polymerase:
TaqDNA Polymerase
Product Type:
DNA Polymerase
For Use With (Application):
Standard PCR
Fidelity (vs. Taq):
1X
Overhang:
3'-A
Starting Material:
DNA
Content And Storage: •TaqDNA Polymerase (5 U/μL), 20 μL• 10XTaqBuffer with KCl, 0.6 mL• 10XTaqBuffer with (NH4)2SO4, 0.6 mL• 25 mM MgCl2, 0.6 mLStore at -20°C.
GC-Rich PCR Performance: Low
Reaction Speed: Standard
Quantity: 100 Units
Concentration: 5 U/μL
Hot Start: No
Reaction Format: Separate Components
Volume: __
Format: __
Polymerase: TaqDNA Polymerase
Product Type: TaqDNA Polymerase (Recombinant)
For Use With (Application): Standard PCR
Fidelity (vs. Taq): 1X
Overhang: 3'-A
Starting Material: __
Content And Storage:
•TaqDNA Polymerase (5 U/μL), 20 μL• 10XTaqBuffer with KCl, 0.6 mL• 10XTaqBuffer with (NH4)2SO4, 0.6 mL• 25 mM MgCl2, 0.6 mLStore at -20°C.
GC-Rich PCR Performance:
Low
Reaction Speed:
Standard
Quantity:
100 Units
Concentration:
5 U/μL
Hot Start:
No
Reaction Format:
Separate Components
Volume:
__
Format:
__
Polymerase:
TaqDNA Polymerase
Product Type:
TaqDNA Polymerase (Recombinant)
For Use With (Application):
Standard PCR
Fidelity (vs. Taq):
1X
Overhang:
3'-A
Starting Material:
__
Content And Storage: •TaqDNA Polymerase (5 U/μL), 100 μL• 10XTaqBuffer with KCl, 2 x 1.25 mL• 10XTaqBuffer with (NH4)2SO4, 2 x 1.25 mL• 25 mM MgCl2, 2 x 1.25 mLStore at -20°C.
GC-Rich PCR Performance: Low
Reaction Speed: Standard
Quantity: 500 Units
Concentration: 5 U/μL
Hot Start: No
Reaction Format: Separate Components
Volume: __
Format: __
Polymerase: TaqDNA Polymerase
Product Type: TaqDNA Polymerase (Recombinant)
For Use With (Application): Standard PCR
Fidelity (vs. Taq): 1X
Overhang: 3'-A
Starting Material: __
Content And Storage:
•TaqDNA Polymerase (5 U/μL), 100 μL• 10XTaqBuffer with KCl, 2 x 1.25 mL• 10XTaqBuffer with (NH4)2SO4, 2 x 1.25 mL• 25 mM MgCl2, 2 x 1.25 mLStore at -20°C.
GC-Rich PCR Performance:
Low
Reaction Speed:
Standard
Quantity:
500 Units
Concentration:
5 U/μL
Hot Start:
No
Reaction Format:
Separate Components
Volume:
__
Format:
__
Polymerase:
TaqDNA Polymerase
Product Type:
TaqDNA Polymerase (Recombinant)
For Use With (Application):
Standard PCR
Fidelity (vs. Taq):
1X
Overhang:
3'-A
Starting Material:
__
Content And Storage: •TaqDNA Polymerase (1 U/μL), 500 μL• 10XTaqBuffer with KCl, 2 x 1.25 mL• 10XTaqBuffer with (NH4)2SO4, 2 x 1.25 mL• 25 mM MgCl2, 2 x 1.25 mLStore at -20°C.
GC-Rich PCR Performance: Low
Reaction Speed: Standard
Quantity: 500 Units
Concentration: 1 U/μL
Hot Start: No
Reaction Format: Separate Components
Volume: __
Format: __
Polymerase: TaqDNA Polymerase
Product Type: TaqDNA Polymerase (recombinant)
For Use With (Application): Standard PCR
Fidelity (vs. Taq): 1X
Overhang: 3'-A
Starting Material: __
Content And Storage:
•TaqDNA Polymerase (1 U/μL), 500 μL• 10XTaqBuffer with KCl, 2 x 1.25 mL• 10XTaqBuffer with (NH4)2SO4, 2 x 1.25 mL• 25 mM MgCl2, 2 x 1.25 mLStore at -20°C.
GC-Rich PCR Performance:
Low
Reaction Speed:
Standard
Quantity:
500 Units
Concentration:
1 U/μL
Hot Start:
No
Reaction Format:
Separate Components
Volume:
__
Format:
__
Polymerase:
TaqDNA Polymerase
Product Type:
TaqDNA Polymerase (recombinant)
For Use With (Application):
Standard PCR
Fidelity (vs. Taq):
1X
Overhang:
3'-A
Starting Material:
__