Thermo Scientific™ RevertAid Reverse Transcriptase (200 U/μL)

Description

• Thermo Scientific RevertAid Reverse Transcriptase (RT) is a recombinant M-MuLV RT
• It differs from the M-MuLV RT by its structure and catalytic properties
• The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity and a RNase H activity specific to RNA in RNA-DNA hybrids that is significantly lower than that of Avian Myeloblastosis Virus (AMV) reverse transcriptase
• Features of RevertAid Reverse Transcriptase include: • Efficient synthesis of full-length first strand cDNA up to 13 kb • Optimum activity at 42°C • Active up to 50°C • Incorporates modified nucleotides (e.g., Cy3-, Cy5-, rhodamine-, aminoallyl-, and fluorescein-labeled nucleotides) Applications • First strand cDNA synthesis for RT-PCR and real-time RT-PCR • Synthesis of cDNA for cloning and expression • Generation of labeled cDNA probes for microarrays • DNA labeling • Analysis of RNA by primer extension Source RevertAid Reverse Transcriptase is sourced from E.coli cells with a cloned fragment of the pol gene encoding Moloney Murine Leukemia Virus reverse transcriptase.