Thermo Scientific™ Maxima H Minus Reverse Transcriptase (200 U/μL)

Description

• Thermo Scientific Maxima H Minus Reverse Transcriptase (RT) was developed through in vitro evolution of M-MuLV RT
• The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity but lacks RNase H activity due to mutation in RNase H domain of M-MuLV RT
• The engineered enzyme features dramatically improved thermostability, 50X higher processivity, robustness, and increased synthesis rate compared to wild type M-MuLV RT
• The eliminated RNase H activity enables the enzyme to produce very long RNA transcripts up to 20 kb
• Due to its high thermostability, the enzyme maintains full activity during the entire reverse transcription reaction and generates high yields of cDNA
• The reaction temperature can be increased to 65°C for efficient transcription of RNA regions with a high secondary structure or to improve specificity using gene specific primers
• The extremely high processivity of Maxima H Minus enzyme results in increased resistance to common reaction inhibitors, such as guanidine, formamide, and ethanol
• Features of Maxima H Minus Reverse Transcriptase include: • Thermostable—90% active after incubation at 50°C for 60 minutes in a reaction mixture • Active up to 65°C • High yields of full-length cDNA up to 20 kb • High sensitivity—reproducible cDNA synthesis from a wide range of starting total RNA amounts (1 pg to 5 μg) • Efficient—complete cDNA synthesis in 15 to 30 minutes • Increased resistance to common reaction inhibitors • Incorporates modified nucleotides Applications • First strand cDNA synthesis for RT-PCR and RT-qPCR • Reverse transcription at elevated temperatures to reduce effects of secondary structure • Synthesis of cDNA for cloning and expression • Generation of labeled cDNA probes for microarrays • Analysis of RNA by primer extension Related products • Maxima H Minus Reverse Transcriptase (Cat
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• EP0752) • Maxima H Minus First Strand cDNA Synthesis Kit (Cat
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• K1652) • Maxima H Minus First Strand cDNA Synthesis Kit, with dsDNase (Cat
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• K1681) • Maxima H Minus cDNA Synthesis Master Mix (Cat
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• M1661) • Maxima H Minus cDNA Synthesis Master Mix, with dsDNase (Cat
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• M1681) Order Info Orders placed Monday through Wednesday ship next day; Thursday and Friday orders ship the following Monday.