FERK0351
Thermo Scientific™ Luminaris Color Probe qPCR Master Mix
Manufacturer: Fischer Scientific
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Content And Storage
Contains:• 2 x 1.25 mL Luminaris Color Probe qPCR Master Mix (2X) containing Hot Start Taq DNA Polymerase, UDG and dNTPs (with dUTP) in an optimized PCR buffer with a blue dye; sufficient for 250 x 20 μL reactions• 1 x 1.25 mL 40X Yellow Sample Buffer• 2 x 1.25 mL Nuclease-Free WaterStore at -20°C.
Detection Method
Primer-probe
GC-Rich PCR Performance
High
Polymerase
Taq DNA Polymerase
For Use With (Equipment)
BioRad CFX96™, BioRad iCycler iQ, BioRad iQ5, BioRad MyiQ, MJ Chromo4, Eppendorf MasterCycler RealPlex, Corbett RotorGene, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Cepheid SmartCycler, MJ Opticon
Product Type
qPCR Master Mix
Sufficient For
250 Reactions of 20 μL
Concentration
2X (Luminaris Color Probe qPCR Master Mix), 40X (Yellow Sample Buffer)
Sample Type
DNA, dsDNA
Description
Color Probe
Format
Tube
PCR Method
qPCR
Reaction Speed
Standard
Product Line
Luminaris
Quantity
250 x 20 μL Reactions
For Use With (Application)
Gene Expression
No. of Reactions
250 Reactions
Volume
2 x 1.25 mL
Related Products
Description
- Luminaris Color Probe and Luminaris Probe qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR
- The master mixes include Thermo Scientific Hot Start Taq DNA polymerase, uracil-DNA glycosylase (UDG) and dNTPs in an optimized PCR buffer
- Only the template, primers and probe need to be added
- The Hot Start Taq DNA polymerase, in combination with an optimized buffer, ensures PCR specificity and sensitivity
- dUTP and UDG are included in the mix for carry-over contamination control
- The Luminaris Color Probe qPCR Master Mixes are supplemented with an inert blue dye and a separate Yellow Sample Buffer that contains a yellow dye
- The qPCR reaction mix containing both components is green
- The use of Luminaris Color Probe and Luminaris Probe qPCR Master Mixes in qPCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates
- Individual formulations of Luminaris Color Probe and Luminaris Probe qPCR Master Mixes with ROX at different concentrations as passive reference dye are available for most qPCR platforms
- Highlights Blue Master Mix and Yellow Sample Buffer for easy pipetting UDG in the master mix to prevent carry-over contamination Excellent qPCR performance Specificity — Hot Start Taq DNA Polymerase and the optimized buffer reduce primer dimers Sensitivitity — single copy detection Wide linear range — accurate quantification across 9 orders of magnitude Reproducibility and convenience — ready-to-use 2X master mix Equal performance of blue and colorless master mixes Versions with premixed ROX for different real-time platforms Standard cycling protocols
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Content And Storage: Contains:• 2 x 1.25 mL Luminaris Color Probe qPCR Master Mix (2X) containing Hot Start Taq DNA Polymerase, UDG and dNTPs (with dUTP) in an optimized PCR buffer with a blue dye; sufficient for 250 x 20 μL reactions• 1 x 1.25 mL 40X Yellow Sample Buffer• 2 x 1.25 mL Nuclease-Free WaterStore at -20°C.
Detection Method: Primer-probe
GC-Rich PCR Performance: High
Polymerase: Taq DNA Polymerase
For Use With (Equipment): BioRad CFX96™, BioRad iCycler iQ, BioRad iQ5, BioRad MyiQ, MJ Chromo4, Eppendorf MasterCycler RealPlex, Corbett RotorGene, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Cepheid SmartCycler, MJ Opticon
Product Type: qPCR Master Mix
Sufficient For: 250 Reactions of 20 μL
Concentration: 2X (Luminaris Color Probe qPCR Master Mix), 40X (Yellow Sample Buffer)
Sample Type: DNA, dsDNA
Description: Color Probe
Format: Tube
PCR Method: qPCR
Reaction Speed: Standard
Product Line: Luminaris
Quantity: 250 x 20 μL Reactions
For Use With (Application): Gene Expression
No. of Reactions: 250 Reactions
Volume: 2 x 1.25 mL
Content And Storage:
Contains:• 2 x 1.25 mL Luminaris Color Probe qPCR Master Mix (2X) containing Hot Start Taq DNA Polymerase, UDG and dNTPs (with dUTP) in an optimized PCR buffer with a blue dye; sufficient for 250 x 20 μL reactions• 1 x 1.25 mL 40X Yellow Sample Buffer• 2 x 1.25 mL Nuclease-Free WaterStore at -20°C.
Detection Method:
Primer-probe
GC-Rich PCR Performance:
High
Polymerase:
Taq DNA Polymerase
For Use With (Equipment):
BioRad CFX96™, BioRad iCycler iQ, BioRad iQ5, BioRad MyiQ, MJ Chromo4, Eppendorf MasterCycler RealPlex, Corbett RotorGene, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Cepheid SmartCycler, MJ Opticon
Product Type:
qPCR Master Mix
Sufficient For:
250 Reactions of 20 μL
Concentration:
2X (Luminaris Color Probe qPCR Master Mix), 40X (Yellow Sample Buffer)
Sample Type:
DNA, dsDNA
Description:
Color Probe
Format:
Tube
PCR Method:
qPCR
Reaction Speed:
Standard
Product Line:
Luminaris
Quantity:
250 x 20 μL Reactions
For Use With (Application):
Gene Expression
No. of Reactions:
250 Reactions
Volume:
2 x 1.25 mL
Content And Storage: Contains:• 4 x 1.25 mL Luminaris Color Probe qPCR Master Mix (2X) containing Hot Start Taq DNA Polymerase, UDG and dNTPs (with dUTP) in an optimized PCR buffer with a blue dye; sufficient for 500 x 20 μL reactions• 1 x 1.25 mL 40X Yellow Sample Buffer• 4 x 1.25 mL Nuclease-Free WaterStore at -20°C.
Detection Method: Primer-probe
GC-Rich PCR Performance: High
Polymerase: Taq DNA Polymerase
For Use With (Equipment): BioRad CFX96™, BioRad iCycler iQ, BioRad iQ5, BioRad MyiQ, MJ Chromo4, Eppendorf MasterCycler RealPlex, Corbett RotorGene, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Cepheid SmartCycler, MJ Opticon
Product Type: qPCR Master Mix
Sufficient For: 500 Reactions of 20 μL
Concentration: 2X (Luminaris Color Probe qPCR Master Mix), 40X (Yellow Sample Buffer)
Sample Type: DNA, dsDNA
Description: Color Probe
Format: Tube
PCR Method: qPCR
Reaction Speed: Standard
Product Line: Luminaris
Quantity: 500 x 20 μL Reactions
For Use With (Application): Gene Expression
No. of Reactions: 500 reactions
Volume: 4 x 12.5 mL
Content And Storage:
Contains:• 4 x 1.25 mL Luminaris Color Probe qPCR Master Mix (2X) containing Hot Start Taq DNA Polymerase, UDG and dNTPs (with dUTP) in an optimized PCR buffer with a blue dye; sufficient for 500 x 20 μL reactions• 1 x 1.25 mL 40X Yellow Sample Buffer• 4 x 1.25 mL Nuclease-Free WaterStore at -20°C.
Detection Method:
Primer-probe
GC-Rich PCR Performance:
High
Polymerase:
Taq DNA Polymerase
For Use With (Equipment):
BioRad CFX96™, BioRad iCycler iQ, BioRad iQ5, BioRad MyiQ, MJ Chromo4, Eppendorf MasterCycler RealPlex, Corbett RotorGene, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Cepheid SmartCycler, MJ Opticon
Product Type:
qPCR Master Mix
Sufficient For:
500 Reactions of 20 μL
Concentration:
2X (Luminaris Color Probe qPCR Master Mix), 40X (Yellow Sample Buffer)
Sample Type:
DNA, dsDNA
Description:
Color Probe
Format:
Tube
PCR Method:
qPCR
Reaction Speed:
Standard
Product Line:
Luminaris
Quantity:
500 x 20 μL Reactions
For Use With (Application):
Gene Expression
No. of Reactions:
500 reactions
Volume:
4 x 12.5 mL
Content And Storage: Contains:• 10 x 1.25 mL Luminaris Color Probe qPCR Master Mix (2X) containing Hot Start Taq DNA Polymerase, UDG and dNTPs (with dUTP) in an optimized PCR buffer with a blue dye; sufficient for 1250 x 20 μL reactions• 3 x 1.25 mL 40X Yellow Sample Buffer• 10 x 1.25 mL Nuclease-Free WaterStore at -20°C.
Detection Method: Primer-probe
GC-Rich PCR Performance: High
Polymerase: Taq DNA Polymerase
For Use With (Equipment): BioRad CFX96™, BioRad iCycler iQ, BioRad iQ5, BioRad MyiQ, MJ Chromo4, Eppendorf MasterCycler RealPlex, Corbett RotorGene, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Cepheid SmartCycler, MJ Opticon
Product Type: qPCR Master Mix
Sufficient For: 1250 Reactions of 20 μL
Concentration: 2X (Luminaris Color Probe qPCR Master Mix), 40X (Yellow Sample Buffer)
Sample Type: DNA, dsDNA
Description: Color Probe
Format: Tube
PCR Method: qPCR
Reaction Speed: Standard
Product Line: Luminaris
Quantity: 1250 x 20 μL Reactions
For Use With (Application): Gene Expression
No. of Reactions: 1,250 Reactions
Volume: 10 x 1.25 mL
Content And Storage:
Contains:• 10 x 1.25 mL Luminaris Color Probe qPCR Master Mix (2X) containing Hot Start Taq DNA Polymerase, UDG and dNTPs (with dUTP) in an optimized PCR buffer with a blue dye; sufficient for 1250 x 20 μL reactions• 3 x 1.25 mL 40X Yellow Sample Buffer• 10 x 1.25 mL Nuclease-Free WaterStore at -20°C.
Detection Method:
Primer-probe
GC-Rich PCR Performance:
High
Polymerase:
Taq DNA Polymerase
For Use With (Equipment):
BioRad CFX96™, BioRad iCycler iQ, BioRad iQ5, BioRad MyiQ, MJ Chromo4, Eppendorf MasterCycler RealPlex, Corbett RotorGene, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Cepheid SmartCycler, MJ Opticon
Product Type:
qPCR Master Mix
Sufficient For:
1250 Reactions of 20 μL
Concentration:
2X (Luminaris Color Probe qPCR Master Mix), 40X (Yellow Sample Buffer)
Sample Type:
DNA, dsDNA
Description:
Color Probe
Format:
Tube
PCR Method:
qPCR
Reaction Speed:
Standard
Product Line:
Luminaris
Quantity:
1250 x 20 μL Reactions
For Use With (Application):
Gene Expression
No. of Reactions:
1,250 Reactions
Volume:
10 x 1.25 mL
Content And Storage: Contains:• 4 x 12.5 mL Luminaris Color Probe qPCR Master Mix (2X) containing Hot Start Taq DNA Polymerase, UDG and dNTPs (with dUTP) in an optimized PCR buffer with a blue dye; sufficient for 5000 x 20 μL reactions• 1 x 13 mL 40X Yellow Sample Buffer• 2 x 30 mL Nuclease-Free WaterStore at -20°C.
Detection Method: Primer-probe
GC-Rich PCR Performance: High
Polymerase: Taq DNA Polymerase
For Use With (Equipment): BioRad CFX96™, BioRad iCycler iQ, BioRad iQ5, BioRad MyiQ, MJ Chromo4, Eppendorf MasterCycler RealPlex, Corbett RotorGene, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Cepheid SmartCycler, MJ Opticon
Product Type: qPCR Master Mix
Sufficient For: 5000 Reactions of 20 μL
Concentration: 2X (Luminaris Color Probe qPCR Master Mix), 40X (Yellow Sample Buffer)
Sample Type: DNA, dsDNA
Description: Color Probe
Format: Tube
PCR Method: qPCR
Reaction Speed: Standard
Product Line: Luminaris
Quantity: 5000 x 20 μL Reactions
For Use With (Application): Gene Expression
No. of Reactions: 5,000 Reactions
Volume: 4 x 12.5 mL
Content And Storage:
Contains:• 4 x 12.5 mL Luminaris Color Probe qPCR Master Mix (2X) containing Hot Start Taq DNA Polymerase, UDG and dNTPs (with dUTP) in an optimized PCR buffer with a blue dye; sufficient for 5000 x 20 μL reactions• 1 x 13 mL 40X Yellow Sample Buffer• 2 x 30 mL Nuclease-Free WaterStore at -20°C.
Detection Method:
Primer-probe
GC-Rich PCR Performance:
High
Polymerase:
Taq DNA Polymerase
For Use With (Equipment):
BioRad CFX96™, BioRad iCycler iQ, BioRad iQ5, BioRad MyiQ, MJ Chromo4, Eppendorf MasterCycler RealPlex, Corbett RotorGene, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Cepheid SmartCycler, MJ Opticon
Product Type:
qPCR Master Mix
Sufficient For:
5000 Reactions of 20 μL
Concentration:
2X (Luminaris Color Probe qPCR Master Mix), 40X (Yellow Sample Buffer)
Sample Type:
DNA, dsDNA
Description:
Color Probe
Format:
Tube
PCR Method:
qPCR
Reaction Speed:
Standard
Product Line:
Luminaris
Quantity:
5000 x 20 μL Reactions
For Use With (Application):
Gene Expression
No. of Reactions:
5,000 Reactions
Volume:
4 x 12.5 mL