Thermo Scientific™ Maxima H Minus First Strand cDNA Synthesis Kit PROMO

Description

• This kit allows synthesis of long cDNAs up to 20 kb at elevated temperatures (up to 65°C), superseding other systems' abilities to produce full-length cDNA
• Due to increased synthesis rates the reaction can be completed in 30 minutes
• For reverse transcription the kit uses Maxima H Minus Reverse Transcriptase (RT), which is an advanced enzyme derived by in vitro evolution of MMLV RT
• The Maxima H Minus First Strand cDNA Synthesis Kit with dsDNase provides a dramatically simplified workflow that combines genomic DNA elimination and cDNA synthesis into one-tube procedure
• The kit contains a novel double-strand-specific DNase (dsDNase) engineered to remove contaminating genomic DNA from RNA preps in two minutes without damage to quality or quantity of RNA
• Highly specific dsDNase activity towards double-stranded DNA ensures that single-stranded DNA (such as cDNA and primers) is not cleaved, and dsDNase treated RNA can be directly added to reverse transcription
• Features of the Maxima H Minus First Strand cDNA Synthesis Kit include: Increased reaction temperatures–the first strand of cDNA can be synthesized within the 42-65°C temperature range High yields of full-length first strand cDNA–with RNA templates up to 20 kb Flexible priming–oligo(dT) 18 , random hexamer or gene-specific primers Integrated genomic DNA removal step with dsDNase kit Applications First Strand cDNA synthesis for RT-PCR Construction of cDNA libraries Generation of probes for hybridization Antisense RNA synthesis Contents Maxima H Minus Enzyme Mix Oligo(dT) 18 and Random hexamer primers 5X RT Buffer dNTP Mix Nuclease-free water First Strand Synthesis Kit with dsDNase also contains dsDNase and 10X dsDNase Buffer Additional information about reaction components Maxima H Minus Enzyme Mix contains Maxima H Minus Reverse Transcriptase and RiboLock RNase Inhibitor
• RiboLock RNase Inhibitor effectively protects RNA templates from degradation by RNases A, B, and C at temperatures up to 55°C
• Oligo(dT) 18 and random hexamer primers are supplied with the kit
• Random hexamer primers bind non-specifically and are used to synthesize cDNA from all RNAs in a total RNA population
• The oligo(dT)18 primer selectively anneals to the 3'-end of poly(A) RNA, synthesizing cDNA only from poly(A) tailed mRNA
• Gene-specific primers may also be used with the kit to prime synthesis from a specified sequence
• 10 mM dNTP Mix is a premixed aqueous solution of dATP, dTTP, dCTP, and dGTP
• Nuclease-free water is provided for reaction set-up and dilution of sample DNA
• The absence of endo-, exodeoxyribonucleases, ribonucleases, and phosphatases has been confirmed by appropriate quality tests.