Thermo Scientific™ dNTP/dUTP Mix

Description

• Thermo Scientific dNTP/dUTP Mix is a solution containing dATP, dCTP and dGTP, each at a final concentration of 2 mM and dUTP of 4 mM
• All nucleotides have greater than 99% purity and are free of nuclease activities and human and E
• coli DNA
• The mix offers the possibility to reduce the number of pipetting steps and the risk of reaction set up errors
• dNTP/dUTP Mix is designed for direct use in PCR with Taq DNA Polymerase, reverse transcription, and a wide variety of molecular applications
• Features • Greater than 99% purity confirmed by HPLC • Free of human and E
• coli DNA High stability • Stable for a minimum 100 freeze-thaw cycles • Up to 95% of dNTPs remain in triphosphate form even after 7 weeks at room temperature • Up to 90% of dNTPs remain in triphosphate form after 30 cycles of PCR (1 min at 94°C; 3 min at 72°C) Applications • cDNA synthesis and RT-PCR • PCR, qPCR • Standard PCR • DNA Sequencing Note: dNTP/dUTP Mix is not suitable for PCR with proofreading enzymes or enzyme mixes containing proofreading enzymes.