Contains a specialized, thermostable enzyme isolated from the thermophilic bacterium Thermus aquaticus
The recombinant form of this enzyme is expressed in E
coli
Catalyzes oligonucleotide primer-driven, DNA-template-dependent incorporation of dNTPs into complimentary DNA strands Displays both 5' to 3' polymerase and exonuclease activities Used for the DNA polymerase chain reaction (PCR) in order to amplify DNA sequences Other application include RT-PCR, DNA sequencing and DNA labeling