N8080247
Applied Biosystems™ AmpliTaq Gold™ DNA Polymerase with Buffer II and MgCl2
Manufacturer: Fischer Scientific
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | N8080247-Each-of-1 | In Stock | ₹ 1,03,356.48 |
N8080247 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 1,03,356.48
GST (18%): ₹ 18,604.166
Total Price: ₹ 1,21,960.646
Content And Storage
• AmpliTaq Gold DNA Polymerase (5 U/μL), 200 μL• 10X PCR Buffer II, 4 x 1.5 mL• 25 mM MgCl2, 4 x 1.5 mLStore at -15°C to -30°C.
GC-Rich PCR Performance
Low
Reaction Speed
Standard
Product Type
DNA Polymerase
Shipping Condition
Dry Ice
Concentration
5 U/μL
Hot Start
Built-In Hot Start
Overhang
3'-A
Size (Final Product)
5 kb or less
Format
Tube
Polymerase
AmpliTaq Gold DNA Polymerase
Exonuclease Activity
5' - 3'
Quantity
1000 Units
For Use With (Application)
Hot-start PCR
Fidelity (vs. Taq)
1X
No. of Reactions
800 Reactions
Reaction Format
Separate Components
Starting Material
DNA
Related Products
Description
- AmpliTaq Gold DNA Polymerase with Buffer II and MgCl 2 is a chemically modified form of AmpliTaq DNA Polymerase requiring thermal activation
- The modified enzyme is provided in an inactive state and upon thermal activation, the modifier is permanently released, regenerating active enzyme
- The resulting hot-start PCR amplification provides increased sensitivity, specificity, and yield over conventional PCR techniques
- AmpliTaq Gold DNA Polymerase can be activated partially or completely in a pre-PCR heat step or can be allowed to activate slowly in a time-released manner during the denaturation steps of thermal cycling
- With or without a limited up-front heat activation step, active enzyme is released slowly during thermal cycling to match template concentration and increase specificity
- The yield of specific product increases as reactants are not wasted in the formation of unintended products
- Features Automated chemical hot-start enzyme for increased specificity, sensitivity, and yield Time-released thermal activation improves sensitivity in low copy number amplifications Successful multiplex PCR saves time and reagents Includes GeneAmp 10X PCR Buffer II with MgCl 2 Hot-start technology reduces risk of biological contamination Notes For better performance AmpliTaq Gold 360 DNA Polymerase is purified by an additional proprietary separation process to eliminate contaminating bacterial DNA sequences from the enzyme preparation
- When used with the enhanced 360 Gold Buffer, this ultra-pure enzyme reduces false positive results, amplifies low-level target sequences, and promotes the amplification of a variety of templates
- Order Info Shipping condition: Dry Ice
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Content And Storage: • AmpliTaq Gold DNA Polymerase (5 U/μL), 200 μL• 10X PCR Buffer II, 4 x 1.5 mL• 25 mM MgCl2, 4 x 1.5 mLStore at -15°C to -30°C.
GC-Rich PCR Performance: Low
Reaction Speed: Standard
Product Type: DNA Polymerase
Shipping Condition: Dry Ice
Concentration: 5 U/μL
Hot Start: Built-In Hot Start
Overhang: 3'-A
Size (Final Product): 5 kb or less
Format: Tube
Polymerase: AmpliTaq Gold DNA Polymerase
Exonuclease Activity: 5' - 3'
Quantity: 1000 Units
For Use With (Application): Hot-start PCR
Fidelity (vs. Taq): 1X
No. of Reactions: 800 Reactions
Reaction Format: Separate Components
Starting Material: DNA
Content And Storage:
• AmpliTaq Gold DNA Polymerase (5 U/μL), 200 μL• 10X PCR Buffer II, 4 x 1.5 mL• 25 mM MgCl2, 4 x 1.5 mLStore at -15°C to -30°C.
GC-Rich PCR Performance:
Low
Reaction Speed:
Standard
Product Type:
DNA Polymerase
Shipping Condition:
Dry Ice
Concentration:
5 U/μL
Hot Start:
Built-In Hot Start
Overhang:
3'-A
Size (Final Product):
5 kb or less
Format:
Tube
Polymerase:
AmpliTaq Gold DNA Polymerase
Exonuclease Activity:
5' - 3'
Quantity:
1000 Units
For Use With (Application):
Hot-start PCR
Fidelity (vs. Taq):
1X
No. of Reactions:
800 Reactions
Reaction Format:
Separate Components
Starting Material:
DNA
Content And Storage: • AmpliTaq Gold DNA Polymerase (5 U/μL), 5 x 200 μL• 10X PCR Buffer I (with MgCl2), 20 x 1.5 mLStore at -15°C to -30°C.
GC-Rich PCR Performance: Low
Reaction Speed: Standard
Product Type: DNA Polymerase With Buffer I
Shipping Condition: Dry Ice
Concentration: 5 U/μL
Hot Start: Built-In Hot Start
Overhang: 3'-A
Size (Final Product): 5 kb or less
Format: Tube
Polymerase: AmpliTaq Gold DNA Polymerase
Exonuclease Activity: 5' - 3'
Quantity: 5 x 1000 Units
For Use With (Application): Hot-start PCR
Fidelity (vs. Taq): 1X
No. of Reactions: 4000 Reactions
Reaction Format: Separate Components
Starting Material: DNA
Content And Storage:
• AmpliTaq Gold DNA Polymerase (5 U/μL), 5 x 200 μL• 10X PCR Buffer I (with MgCl2), 20 x 1.5 mLStore at -15°C to -30°C.
GC-Rich PCR Performance:
Low
Reaction Speed:
Standard
Product Type:
DNA Polymerase With Buffer I
Shipping Condition:
Dry Ice
Concentration:
5 U/μL
Hot Start:
Built-In Hot Start
Overhang:
3'-A
Size (Final Product):
5 kb or less
Format:
Tube
Polymerase:
AmpliTaq Gold DNA Polymerase
Exonuclease Activity:
5' - 3'
Quantity:
5 x 1000 Units
For Use With (Application):
Hot-start PCR
Fidelity (vs. Taq):
1X
No. of Reactions:
4000 Reactions
Reaction Format:
Separate Components
Starting Material:
DNA
Content And Storage: • AmpliTaq Gold DNA Polymerase (5 U/μL), 5 x 200 μL• 10X PCR Buffer II, 20 x 1.5 mL• 25 mM MgCl2, 20 x 1.5 mLStore at -15°C to -30°C.
GC-Rich PCR Performance: Low
Reaction Speed: Standard
Product Type: DNA Polymerase
Shipping Condition: Dry Ice
Concentration: 5 U/μL
Hot Start: Built-In Hot Start
Overhang: 3'-A
Size (Final Product): 5 kb or less
Format: Tube
Polymerase: AmpliTaq Gold DNA Polymerase
Exonuclease Activity: 5' - 3'
Quantity: 5000 Units
For Use With (Application): Hot-start PCR
Fidelity (vs. Taq): 1X
No. of Reactions: 4000 Reactions
Reaction Format: Separate Components
Starting Material: DNA
Content And Storage:
• AmpliTaq Gold DNA Polymerase (5 U/μL), 5 x 200 μL• 10X PCR Buffer II, 20 x 1.5 mL• 25 mM MgCl2, 20 x 1.5 mLStore at -15°C to -30°C.
GC-Rich PCR Performance:
Low
Reaction Speed:
Standard
Product Type:
DNA Polymerase
Shipping Condition:
Dry Ice
Concentration:
5 U/μL
Hot Start:
Built-In Hot Start
Overhang:
3'-A
Size (Final Product):
5 kb or less
Format:
Tube
Polymerase:
AmpliTaq Gold DNA Polymerase
Exonuclease Activity:
5' - 3'
Quantity:
5000 Units
For Use With (Application):
Hot-start PCR
Fidelity (vs. Taq):
1X
No. of Reactions:
4000 Reactions
Reaction Format:
Separate Components
Starting Material:
DNA
Content And Storage: • AmpliTaq Gold DNA Polymerase (5 U/μL), 25 x 200 μL• 10X PCR Buffer I (with MgCl2), 100 x 1.5 mLStore at -15°C to -30°C.
GC-Rich PCR Performance: Low
Reaction Speed: Standard
Product Type: DNA Polymerase With Buffer I
Shipping Condition: Dry Ice
Concentration: 5 U/μL
Hot Start: Built-In Hot Start
Overhang: 3'-A
Size (Final Product): 5 kb or less
Format: Tube
Polymerase: AmpliTaq Gold DNA Polymerase
Exonuclease Activity: 5' - 3'
Quantity: 25 x 1000 Units
For Use With (Application): Hot-start PCR
Fidelity (vs. Taq): 1X
No. of Reactions: 20,000 Reactions
Reaction Format: Separate Components
Starting Material: DNA
Content And Storage:
• AmpliTaq Gold DNA Polymerase (5 U/μL), 25 x 200 μL• 10X PCR Buffer I (with MgCl2), 100 x 1.5 mLStore at -15°C to -30°C.
GC-Rich PCR Performance:
Low
Reaction Speed:
Standard
Product Type:
DNA Polymerase With Buffer I
Shipping Condition:
Dry Ice
Concentration:
5 U/μL
Hot Start:
Built-In Hot Start
Overhang:
3'-A
Size (Final Product):
5 kb or less
Format:
Tube
Polymerase:
AmpliTaq Gold DNA Polymerase
Exonuclease Activity:
5' - 3'
Quantity:
25 x 1000 Units
For Use With (Application):
Hot-start PCR
Fidelity (vs. Taq):
1X
No. of Reactions:
20,000 Reactions
Reaction Format:
Separate Components
Starting Material:
DNA