Description Optimized to quantitatively amplify a two-color duplex, enabling the user to amplify a transcript of interest as well as a reference gene in a single reaction Achieves sensitive real-time detection and relative quantification of RNA expression levels for monitoring of stem cell differentiation in a multiplexed amplification Quantitatively amplifies a two-color duplex Amplifies in a single tube the transcript of interest and reference transcript (GAPDH and Actb) Reduces the number of reactions required for the measurement of multiple transcripts Improves experimental data quality by measuring all the transcripts in the same well