Applied Biosystems™ Primer Express™ Software v3.0.1 License

Description

• The Primer Express™ Software v3.0.1 allows you to design your own primers and probes using TaqMan™ and SYBR™ Green I dye chemistries for gene quantitation and allelic discrimination (SNP) real-time PCR applications
• Developed specifically for use with our StepOne™, StepOnePlus™, 7300, 7500, 7500 Fast, 7900HT, ViiA™ 7, and QuantStudio™ real-time PCR systems, Primer Express™ Software provides customized application-specific documents for absolute⁄relative quantitation and allelic discrimination
• Enables individual oligonucleotide design for real-time PCR applications
• Supports assays based on TaqMan™ and SYBR™ Green I dye chemistries
• Provides design flexibility, ease-of-use, and requires minimal optimization
• Provides robust assay performance when used in accordance with Rapid Assay Development Guidelines (RADG)
• Easy-to-Learn Software The Primer Express™ Software v3.0.1 is flexible, easy to learn and to use, and requires minimal optimization
• It includes an algorithm optimized specifically for use with TaqMan™-based reagents, recommended primer⁄probe concentrations, and default thermal cycling conditions
• Primer Express™ Software also supports assays based on the SYBR™ Green I dye
• This minimizes the need for assay optimization and streamlines the design process
• The software also allows customized designs if the probe and⁄or one or more primers are known
• Default and adjustable parameters (Figure 1) provide the flexibility required for all users, regard Simple to Use Simplicity and flexibility are the hallmarks of Primer Express™ Software v3.0.1, which is fully compatible with all StepOne™, StepOnePlus™, 7300, 7500, 7500 Fast, 7900HT, ViiA™ 7, and QuantStudio™ real-time PCR systems
• Whether your research includes gene quantitation or allelic discrimination studies, Primer Express™ Software belongs in your lab
• Automated and Manual Primer⁄Probe Design The Primer Express™ Software v3.0.1 will design your primers and probes automatically, or you can design them manually
• For automated design, simply choose either TaqMan ™ or TaqMan™ MGB assays
• If you know the sequence, you need only to import (or copy and paste) the sequence and quickly view your results
• Alternatively, if you prefer to design the primers and probes manually, the procedure is equally simple and straight-forward as well
• Rapid Assay Development Guidelines The Primer Express ™ Software v3.0.1 was designed with our Rapid Assay Development Guidelines (RADG)
• The RADG are a simple yet comprehensive set of guidelines for use with our real-time PCR instruments and reagents
• Many traditional variables, including magnesium chloride concentration and the thermal cycling protocol itself, have been standardized, which substantially reduces assay development time
• For Research Use Only
• Not for use in diagnostics procedures.