BugBuster® Master Mix

Description

• General description: The BugBuster® Master Mix enables efficient protein purification and other related applications. It replaces the need for mechanical methods like the French press or sonication. The two available package sizes contain sufficient reagents for extracting proteins from either 20 grams or 100 grams of cell paste.
• Application: BugBuster® Master Mix has been used:to resuspend and lyse the cell pellets during protein purification[1]to extract proteins for immunoblotting[2]as a bacterial lysis buffer for the preparation of inclusion bodies[3]
• Biochem/physiol Actions: BugBuster® Protein Extraction Reagent is specifically designed to gently disrupt the cell wall of E. coli, allowing the release of soluble proteins. This unique formulation utilizes a detergent mix that can effectively perforate the cell wall without denaturing the soluble proteins. By combining BugBuster Reagent, rLysozyme solution, and Benzonase Nuclease, our BugBuster Master Mix enhances protein extraction efficiency and simplifies downstream processing of protein extracts.
• Features and Benefits: Efficient lysis of Gram-positive and Gram-negative bacteriaMaximum recovery of active, soluble proteinCapable of cell wall perforation without denaturing soluble proteinUseful for the preparation of high purity inclusion bodiesCompatible with protease and phosphatase inhibitor cocktail
• Warning: Toxicity: Standard Handling (A)
• Legal Information: BUGBUSTER is a registered trademark of Merck KGaA, Darmstadt, Germany