Thermo Scientific™ Peptide Retention Standard

Description

• Thermo Scientific™ Pierce Peptide Retention Standard is a mixture of five c-terminal amide decapeptides that provide reference elution profiles in reverse-phase HPLC
• Retention times are predicted by totaling the values that represent the contribution in minutes of each amino acid residue and the peptide terminal groups
• Retention time is dependent upon the molecular weight of the peptide
• The effect on retention is relatively unimportant with a small peptide, but it increases with the size of the molecule
• The accuracy of predicting peptide retention time significantly decreases beyond 20 residues
• To ensure accuracy, a peptide standard is used to correct for instrument variation, column aging, n-alkyl chain length variation and ligand density
• Highlights: Save time in peptide purification Simplify identification of specific peptides in a complex mixture Increase the efficiency of predicting peptide elution profiles Determine the relative order of peptide elution of a complex mixture Predict the HPLC retention time for peptides of known amino acid composition on reverse phase HPLC columns Monitor column performance: efficiency, selectivity and resolution during column aging Compare reverse-phase columns from different manufacturers Evaluate reverse-phase supports of varying n-alkyl chain lengths and ligand densities