Filter

Adsorption Kit

Agarose

Ammonium Sulfate Solution

Antibody Clean-up Kit

Antibody Purification Kit

Ascites Conditioning Reagent

Complete H37Ra

Freund's Complete Adjuvant

Freund's Complete Adjuvant, Modified, Mycobacterium butyricum

Freund's Incomplete Adjuvant

Freund's Incomplete Adjuvant, Modified

GammaBind Plus Sepharose

Gel Purification Buffer

Gel Regenerant

Gel Spin Plate Kit

IgG Purification Kit

IgM Purification Kit

Imject Blue Carrier Protein

Mycobacterium butyricum

Mycobacterium tuberculosis H37Ra

15°C to 30°C

2°C to 8°C

2°C to 8°C, 20% Ethanol

20°C to 25°C

4°C

4°C to 30°C, 20% Ethanol

4°C. Do not freeze.

4°C. Shipped on ice.

Kit Contents:• Melon Gel 96-Well Spin Plates, 2 each, contains 100 μL of Melon Gel⁄well (Purification Capacity: 1 mg of human IgG⁄well)• Melon Gel Purification Buffer, 2 × 100 mL• Wash Plates (white), 2 each• Collection PlateStorage: Upon receipt store at 4°C. Product shipped at ambient temperature.

Store in cool, dry conditions in well sealed containers, away from oxidizing agents.

Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: 2°C to 8°C.

Store in original container protected from direct sunlight in a dry, cool and well-ventilated area.

Store the unopened product at room temperature. Do not use past expiration date.

Sufficient For: 10 antibody samples, each containing 0.25 to 4 mg IgG• Pierce Ficin Agarose, 2.5 mL• Cysteine-HCl, 1 g• Mouse IgG1 Digestion Buffer, 120 mL• NAb Protein A Plus Spin Column, 1 mL, 1 column• Protein A Binding Buffer, 120 mL• IgG Elution Buffer, 120 mL• Zeba Desalt Spin Columns, 7K MWCO, 2 mL, 10 columns• Spin Columns, 0.8 mL, 10 columns• Microcentrifuge tubes, 2 mL, 30 tubesStore at 4–8°C.

Sufficient For: 10 antibody samples, each containing 0.25 to 4 mg IgG• Pierce Papain Agarose, 1.25 mL• Cysteine-HCl, 1 g• Fab Digestion Buffer, 120 mL• NAb Protein A Plus Spin Column, 1 mL, 1 column• PBS Packs (each makes 500 mL), 2 packs• IgG Elution Buffer, 120 mL• Zeba Desalt Spin Columns, 7K MWCO, 2mL, 10 columns• Spin Columns, 0.8 mL, 10 columns• Microcentrifuge Tubes, 2 mL, 30 tubesStore at 4–8°C.

Sufficient For: 10 antibody samples, each containing 0.25 to 4 mg IgG• Pierce Pepsin Agarose, 1.25 mL• F(ab')2 Digestion Buffer, 120 mL• NAb Protein A Plus Spin Column, 1 mL, 1 column• PBS Packs (each makes 500 mL), 2 packs• IgG Elution Buffer, 120 mL• Zeba Desalt Spin Columns, 7K MWCO, 2 mL, 10 columns• Spin Columns, 0.8mL, 10 columns• Microcentrifuge Tubes, 2 mL, 30 tubesStore at 4–8°C

Sufficient For: 10 antibody samples, each containing 25 to 250 μg IgG• Pierce Ficin Agarose, 0.8 mL• Cysteine-HCl, 1 g• Mouse IgG1 Digestion Buffer, 120 mL• NAb Protein A Plus Spin Columns, 0.2 mL, 2 columns• Protein A Binding Buffer, 50 mL• IgG Elution Buffer, 50 mL• Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, 10 columns• Spin Columns, 0.8 mL, 10 columns• Microcentrifuge tubes, 2 mL, 30 tubesStore at 4–8°C.

Sufficient For: 10 antibody samples, each containing 25 to 250 μg IgG• Pierce Papain Agarose, 0.5 mL• Cysteine-HCl, 1 g• Fab Digestion Buffer, 55 mL• NAb Protein A Plus Spin Columns, 0.2 mL, 2 columns• PBS Packs (each makes 500 mL), 2 packs• IgG Elution Buffer, 50 mL• Zeba Desalt Spin Columns, 7K MWCO, 0.5 mL, 10 columns• Spin Columns, 0.8 mL, 10 columns• Microcentrifuge Tubes, 2 mL, 30 tubesStore at 4–8°C.

Sufficient For: 10 antibody samples, each containing 25 to 250 μg IgG• Pierce Pepsin Agarose, 0.5 mL• F(ab')2 Digestion Buffer, 55 mL• NAb Protein A Plus Spin Columns, 0.2 mL, 2 columns• PBS Packs (each makes 500 mL), 2 packs• IgG Elution Buffer, 50 mL• Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, 10 columns• Spin Columns, 0.8 mL, 10 columns• Microcentrifuge Tubes, 2 mL, 30 tubesStore at 4°C

Sufficient For: 10 IgM purifications, each from 0.5 mL mouse ascites samples• MBP Agarose Column, 5 mL, 1 column• IgM Binding Buffer, 800 mL• IgM Elution Buffer, 500 mL• MBP Column Preparation Buffer, 50 mL• Column ExtenderStore at 4°C

Sufficient For: 10 isotype determinations• Isotyping Cassettes (three per pouch), 10 pouches• Sample Diluent, 45 mLStore kit at 2–8°C.

Sufficient For: 10 isotype determinations• Isotyping Cassettes (two per pouch), 10 pouches• Sample Diluent, 45 mLStore kit at 2–8°C.

Sufficient For: 5 conjugation reactions and complete immunogen preparations, each with up to 2 mg sulfhydryl-containing peptide• Imject Maleimide-Activated BSA, 5 x 2 mg• Imject Maleimide Conjugation Buffer, 30 mL• Imject Purification Buffer Salts, 5 x 0.84 g• Zeba Spin Desalting Columns, 7K MWCO, 2 mL, 5 columnsStore the Maleimide-Activated blue Carrier Protein at -20°C. Store remaining components at 4°C.

Sufficient For: 5 conjugation reactions and complete immunogen preparations, each with up to 2 mg sulfhydryl-containing peptide• Imject Maleimide-Activated mcKLH, 5 x 2 mg• Imject Maleimide Conjugation Buffer, 30 mL• Imject Purification Buffer Salts, 5 x 0.84 g• Zeba Spin Desalting Columns, 7K MWCO, 2 mL, 5 columnsStore the Maleimide-Activated blue Carrier Protein at -20°C. Store remaining components at 4°C.

Sufficient For: 5 EDC conjugation reactions and complete immunogen preparations, each with up to 2 mg peptide• Imject Blue Carrier Protein (in MES buffer), 5 x 2 mg• Imject EDC Conjugation Buffer, 30 mL• EDC Crosslinker, 5 x 10 mg• Imject Purification Buffer Salts, 5 x 0.84 g• Zeba Spin Desalting Columns, 7K MWCO, 2 mL, 5 columnsStore at 4°C

Sufficient For: 5 EDC conjugation reactions and complete immunogen preparations, each with up to 2 mg peptide• Imject BSA (in MES buffer), 5 x 2 mg• Imject EDC Conjugation Buffer, 30 mL• EDC Crosslinker, 5 x 10 mg• Imject Purification Buffer Salts, 5 x 0.84 g• Zeba Spin Desalting Columns, 7K MWCO, 2 mL, 5 columnsStore at 4°C.

Sufficient For: 5 EDC conjugation reactions and complete immunogen preparations, each with up to 2 mg peptide• Imject Mariculture KLH (in MES buffer), 5 x 2 mg• Imject EDC Conjugation Buffer, 30 mL• EDC Crosslinker, 5 x 10 mg• Imject Purification Buffer Salts, 5 x 0.84 g• Zeba Spin Desalting Columns, 7K MWCO, 2 mL, 5 columnsStore at 4°C.

Sufficient For: 60 isotype determinations (8 wells per test sample)• Pre-coated 96-well Isotyping Plates, 5 plates• Goat Anti-Mouse IgG+IgA+IgM-HRP Conjugate, 30 mL• TMB Substrate, 55 mL• Stop Solution, 55 mL• 30X Wash Buffer, 200 mL• TBS Buffer Packs (each makes 500 mL), 2 packsStore at 4°C.

Sufficient For: 96 total tests (including 9 standards per use)• Goat Anti-Human IgG (gamma chain) Sensitized Polystyrene Beads, 2mL• Easy-Titer Dilution Buffer, 30 mL• Easy-Titer Blocking Buffer, 15 mLStore at 4°C (do not freeze).

Sufficient For: 96 total tests (including 9 standards per use)• Goat Anti-Human IgG Sensitized Polystyrene Beads, 2mL• Easy-Titer Dilution Buffer, 30 mL• Easy-Titer Blocking Buffer, 15 mLStore at 4°C (do not freeze).

Sufficient For: 96 total tests (including 9 standards per use)• Goat Anti-Human IgM Sensitized Polystyrene Beads, 2mL• Easy-Titer Dilution Buffer, 30 mL• Easy-Titer Blocking Buffer, 15 mLStore at 4°C (do not freeze).

Sufficient For: 96 total tests (including 9 standards per use)• Goat Anti-Mouse IgG Sensitized Beads, 2mL• Easy-Titer Dilution Buffer, 30 mL• Easy-Titer Blocking Buffer, 15 mLStore at 4°C (do not freeze).

Sufficient For: 96 total tests (including 9 standards per use)• Goat Anti-Rabbit IgG Sensitized Beads, 2mL• Easy-Titer Dilution Buffer, 30 mL• Easy-Titer Blocking Buffer, 15 mLStore at 4°C (do not freeze).

Sufficient For: IgG purification from 75 to 150 mL of serum (total approx. 2 g IgG from serum)• Melon Gel Support, 25 mL• Melon Gel Purification Buffer (dry mix to make 100X), 1 L• Melon Gel Regenerant (dry mix to make 1X), 1 LStore at 4°C

Sufficient For: IgG purification from approx. 25 x 0.1 mL serum samples (total approx. 25 mg IgG from serum)• Melon Gel Support, 3 mL• Melon Gel Purification Buffer, 100 mL• Spin Columns, 0.8 mL, 27 columns• Microcentrifuge Tubes, 2 mL, 30 tubesStore at 4°C

Sufficient For: IgG purification from up to 1 L of cell culture supernatant or up to 200 mL of ascites fluid• Melon Gel Support, 200 mL• Melon Gel Purification Buffer (dry mix to make 100X), 1 L• Melon Gel Regenerant (dry mix to make 5X), 1 LStore at 4°C

Sufficient For: Purifying 10 x 0.5 mL IgG samples• Melon Gel IgG Purification Support, 1.2 mL• Melon Gel Purification Buffer, 100 mL• Zeba Spin Desalting Columns, 7K MWCO, 2 mL, 10 columns• Spin Columns, 0.8 mL, 10 columns• Microcentrifuge Tubes, 2 mL, 30 tubesStore at 4°C

Sufficient For: Purifying up to 60 mg immunoglobulin per column• Thiophilic Adsorbent Columns, 3 mL, 4 columns• Binding Buffer, 1 L• Elution Buffer, 1 L• Column Storage Buffer (2X), 100 mL• Guanidine-HCl Crystals, 230 g• Column AccessoriesStore at 4°C.

Upon receipt store at 4°C

Upon receipt store at ambient temperature.

Upon receipt store product at -20°C. Product is shipped with an ice pack.

Upon receipt store product at 4°C. Product is shipped at ambient temperature.

Upon receipt store product at 4°C. Product is shipped at ambient temperature. Do not freeze product.

1 Purification

3 Purifications

1 Column

1 g

1 kg

1 L

1 mg

1 Pack

1 Set

1 Test

1 x 4.7 mL

10 Antibody Samples Each Containing 0.25 to 4 mg IgG

10 Antibody Samples Each Containing 25 to 250 μg IgG

10 Antibody Samples, each containing 0.25 to 4 mg IgG

10 Antibody Samples, each containing 25 to 250 μg IgG

10 g

10 mg

10 mL

10 Reactions

10 Tests

100 g

100 mg

16

2 mg

2 Plates

2.5 mL

20 mg

200 mL

25 g

25 mg

25 mL

250 g

3 mL

3 Tests

30 mL

4 Columns

5 g

5 mL

5 x 10 mL

5 x 2 mg

5 x 2 mg Kit

5 x 20 mg

50 mL

500 g

500 mg

500 mL

6 x 10 mL

6 x 100 mg

60 Tests

96 tests

96 Tests

Complete packsize

Antibody affinity chromatography

ELISA (Antigen Capture), Absorbance

Immunological studies with laboratory animals

Membrane Blot

Solution-based Detection, Absorbance

The antibody to be purified should be in 10 to 50 mL of tissue culture supernatant. Up to 5 mg of antibody can be purified in each run.

Used for the boost injections