Tocris Bioscience™ Tissue Clearing Pro Reagent 2

Description

• Tissue Clearing Pro is a solvent-based tissue clearing technique, comparable to BABB and DISCO
• It does not cause significant shrinkage or tissue damage and does not use as harsh a solvent as other solvent-based techniques
• Tissue Clearing Pro Reagent 2 is part of our Tissue Clearing Pro kit (Cat
• No
• 7389) that can clear a whole mouse brain of up to 8 mm thickness in 24 hours; for sections with a thickness of 1 mm this can be achieved in 2 hours
• For small tissues and thin brain slices (500 μm) that do not require permeabilization, only Tissue Clearing Pro Reagent 1 (Cat
• No
• 7563) is required and tissues can be imaged in Tissue Clearing Pro Reagent 1
• Larger tissues must be placed in Tissue Clearing Pro Reagent 2 to finish clearing, then these samples can be mounted and imaged in Tissue Clearing Pro Reagent 2
• This product is compatible with fluorescent protein, immunolabeling and small molecule staining techniques, as well as being suitable for use with all standard dyes and buffers
• Following 3D imaging, clearing can be reversed for follow-up 2D histology staining
• Tissue Clearing Pro Reagent 2 has a refractive index of 1.53
• Please read the Tissue Clearing Pro Guidebook for more information.