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Cell Culture, Stem Cell Culture

ELISA, Western Blot

Immunoprecipitation

Immunoprecipitation, SDS-Page, Western Blot

Immunoprecipitation, Western Blot

Protein-Protein Interaction, Ligand Binding, ELISA, Immunoprecipitation, 1 D and 2 D Gel Electrophoresis, Western Blot

Ready-to-Use Lysates Are Especially Prepared as Positive Controls for Separation by SDS-PAGE and Subsequent Western Blot Analysis. Lysates Are Prepared in Denaturing Buffer WITHOUT Dissociating Agents (i.e. no 2-Mercaptoethanol or Dithiothreitol Has Been Added). Heat Lysate to 95 C for 5 Minutes and Rapidly Cool. If Dissociating Conditions Are Desired, Add Reducing Agent Prior to Heating. The Recommended Loading Volume / Lane is 10-20 μL Depending on The Size Format of Your Gel.

SDS-Page

SDS-Page, Western Blot

This product is intended for use as a positive control in Western Blot. Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.

Western Blot

Western Blot, In vitro assay

Western Blot, SDS-Page

African Green Monkey

Canine

Hamster

Human

Human, Mouse

Marsupial

Monkey

Mouse

Non-species Specific

Primate

Rat

-20°C

-20°C. Avoid freeze-thaw cycles.

-80°C

-80°C in the dark. Avoid freeze-thaw cycles.

-80°C. Avoid freeze-thaw cycles.

Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Store at -70°C. Avoid freeze-thaw cycles.

Store at -70°C. Avoid freeze/thaw cycles.

Store at -80 C. Avoid freeze-thaw cycles.

Store at -80°C upon receipt. Thaw at room temperature or in a water bath. After thawing, aliquot and store at -20°C or colder. Long term storage at -80°C is recommended. Multiple freeze/thaw cycles are not recommended.

Store at -80°C.

Store at -80°C. Aliquot to avoid repeated freezing and thawing.

Store at -80°C. Aliquot to avoid repeated freezing and thawing. ;Store at -80°C. Aliquot to avoid repeated freezing and thawing.

Store at -80°C. Avoid freeze-thaw cycles.

Store at -80°C. Avoid freeze/thaw cycles.

Store at -80C. Avoid freeze-thaw cycles.

Store at −80°C. Avoid freeze-thaw cycles.

Store at ≤70°C. Avoid freeze/thaw. Ok to freeze.

Store between -60°C and -85°C.

Adaptive Immunity, Angiogenesis, B Cell Development and Differentiation Markers, Breast Cancer, Cancer, Cell Biology, Cellular Markers, Endothelial Cell Markers, Hematopoietic Stem Cell Markers, Hypoxia, Immunology, Innate Immunity, Mast Cell Markers, Mesenchymal Stem Cell Markers, Myeloid Cell Markers, Myeloid derived Suppressor Cell, Neuronal Cell Markers, Neuroscience, Stem Cell Markers, Tumor Suppressors

Adaptive Immunity, Angiogenesis, Breast Cancer, Cancer, Cardiovascular Biology, Neuroscience, Virology Bacteria and Parasites

Adaptive Immunity, Apoptosis, Cancer, HIF Target Genes, Hypoxia, Inflammation, Lipid and Metabolism, Neuroscience, Neurotransmission

Adaptive Immunity, Asthma, Autoimmune Diseases, Autophagy, Cancer, Cytokine Research, Diabetes Research, Immune System Diseases, Immunology, Innate Immunity

Adaptive Immunity, B Cell Development and Differentiation Markers, Cardiovascular Biology, Glycobiology, Immunology, Lipid and Metabolism, Myeloid derived Suppressor Cell, Signal Transduction, Stem Cells

Adaptive Immunity, Cancer, Cytokine Research, GPCR, Growth and Development, Immunology, Innate Immunity, Neuronal Cell Markers, Signal Transduction, Stem Cells

Adaptive Immunity, Checkpoint signaling, Immunology, Stem Cells

Alzheimers Research, Apoptosis, Cancer, Cardiovascular Biology, Cytokine Research, Immunology, Inflammation, Neurodegeneration, Signal Transduction

Angiogenesis, Apoptosis, Breast Cancer, Cancer, Diabetes Research, Hypoxia, Phospho Specific, Protein Kinase, Signal Transduction, Tumor Suppressors

Angiogenesis, Breast Cancer, Cancer, Cell Biology, Cell Cycle and Replication, Dendritic Cell Markers, Neuroscience, Signal Transduction, Transcription Factors and Regulators

Angiogenesis, Cancer

Angiogenesis, Cancer, Cardiovascular Biology, Cell Biology, Cellular Markers, Developmental Biology, Extracellular Matrix, Signal Transduction, Stem Cell Markers

Angiogenesis, Cancer, Cell Cycle and Replication, Cytokine Research, Immunology, Virology Bacteria and Parasites

Angiogenesis, Cancer, Extracellular Matrix

Apoptosis

Apoptosis, Cancer, Cell Biology, Death Receptor Signaling Pathway, Diabetes Research, Hematopoietic Stem Cell Markers, Immunology, Inhibitors Activators and Regulators, Innate Immunity, Signal Transduction, Stem Cells

Apoptosis, Cancer, Cellular Markers, Hypoxia, Neuronal Cell Markers, Neuroscience, Transcription Factors and Regulators, Tumor Suppressors

Apoptosis, Cancer, Diabetes Research, Protein Kinase, Signal Transduction, Transcription Factors and Regulators

Apoptosis, Cancer, Tumor Suppressors

Apoptosis, Cell Cycle and Replication, Immune System Diseases, Immunology, Protein Kinase

Apoptosis, GPCR

Apoptosis, Immunology

Astrocyte Markers, Cancer, Cell Biology, Cellular Markers, Chaperone Mediated Autophagy (CMA), Inflammation, Neurodegeneration, Neuroscience, Signal Transduction, Stem Cell Markers

Autophagy, Cancer, Cardiovascular Biology, Cytokine Research, Immunology, Innate Immunity, Lysosome Markers, Metastasis

Autophagy, Cholesterol Metabolism, Endosome Markers, Glycobiology, Lysosome Markers, Mast Cell Markers, Membrane Trafficking and Chaperones, Virology Bacteria and Parasites

Base Excision Repair, Cancer, DNA Repair

Breast Cancer, Cancer, Cell Cycle and Replication, Checkpoint signaling, Chromatin Research, DNA Double Strand Break Repair, DNA Repair, Homologous Recombination, Non homologous end joining, Tumor Suppressors

Breast Cancer, Cancer, Cell Cycle and Replication, GPCR, Neuronal Cell Markers, Neuroscience, Neurotransmission

Breast Cancer, Cancer, DNA Repair, Protein Kinase

Breast Cancer, Cancer, DNA Repair, Protein Kinase, Signal Transduction

Breast Cancer, Cancer, Protein Kinase

Cancer

Cancer, Cardiovascular Biology

Cancer, Cardiovascular Biology, Cell Biology, Developmental Biology, Virology Bacteria and Parasites

Cancer, Cardiovascular Biology, Cell Cycle and Replication, Cellular Signaling, metabolism

Cancer, Cardiovascular Biology, Cellular Markers, Diabetes Research, Lipid and Metabolism, Membrane Trafficking and Chaperones, Neuroscience, Plasma Membrane Markers, Signal Transduction

Cancer, Cardiovascular Biology, Cellular Markers, Endothelial Cell Markers, Hematopoietic Stem Cell Markers, Immunology, Innate Immunity, Lipid and Metabolism, Stem Cell Markers

Cancer, Cardiovascular Biology, Cellular Signaling, metabolism

Cancer, Cardiovascular Biology, Cellular Signaling, metabolism, Neuroscience

Cancer, Cell Biology, Cell Cycle and Replication, Cellular Markers, Cellular Signaling, Growth and Development, Hypoxia, Phospho Specific, Signal Transduction, Tumor Suppressors, Tyrosine Kinases

Cancer, Cell Cycle and Replication

Cancer, Cell Cycle and Replication, Cytokine Research, Immunology, Innate Immunity

Cancer, Cell Cycle and Replication, GPCR, Neuronal Cell Markers, Neuroscience, Neurotransmission

Cancer, Cellular Markers, Extracellular Matrix, Inflammation, Signal Transduction

Cancer, Cellular Signaling

Cancer, Cellular Signaling, metabolism

Cancer, Diabetes Research, mTOR Pathway, Neuroscience, Signal Transduction

Cancer, DNA replication Transcription Translation and Splicing

Cancer, Epigenetics, metabolism

Cancer, Immunology

Cancer, Immunology, Innate Immunity, Neuroscience

Cancer, Lipid and Metabolism, Neurodegeneration, Neuronal Cell Markers, Neuroscience, Neurotransmission, Phospho Specific, Prostate Cancer

Cancer, metabolism

Cancer, Neuroscience

Cancer, Proteases & Other Enzymes

Cancer, Proteases & Other Enzymes, Signal Transduction

Cancer, Protein Kinase

Cancer, Signal Transduction

Cancer, Stem Cell Markers

Cardiovascular Biology

Cardiovascular Biology, Extracellular Matrix

Cardiovascular Biology, metabolism

Cardiovascular Biology, Neuroscience

Cardiovascular Biology, Signal Transduction

Cell Biology, Cell Cycle and Replication, Cytoskeleton Markers, Loading Controls, Neuroscience

Cell Biology, Cellular Markers, Cytokine Research, Growth and Development, Hematopoietic Stem Cell Markers, Immunology, Innate Immunity, Neuronal Cell Markers, Neuroscience, Stem Cell Markers, Stem Cells

Cell Biology, Cellular Markers, MAP Kinase Signaling, Neuroscience, Stem Cell Markers

Cell Cycle and Replication

Cell Cycle and Replication, Core ESC Like Genes, DNA Repair, DNA replication Transcription Translation and Splicing, Stem Cell Markers

Cell Cycle and Replication, DNA Repair

Cell Cycle and Replication, GPCR

Cell Cycle and Replication, Proteases & Other Enzymes

Cellular Markers, Cellular Signaling, Neuronal Cell Markers

Cellular Markers, Neuronal Cell Markers, Neuroscience

Cellular Markers, Neuronal Cell Markers, Neurotransmission

Cellular Signaling

Cellular Signaling, Neuroscience

Chromatin Research

Cytokine Research

Cytokine Research, GPCR, Signal Transduction

Cytokine Research, Immunology

Cytokine Research, Immunology, Infections (Virus Bacteria and Parasites), Inflammation, Innate Immunity, Signal Transduction, Toll Like Receptors

Cytokine Research, Signal Transduction

Developmental Biology

DNA replication Transcription Translation and Splicing

DNA replication Transcription Translation and Splicing, Epigenetics

DNA replication Transcription Translation and Splicing, metabolism

DNA replication Transcription Translation and Splicing, Neuronal Cell Markers, Neuroscience

DNA replication Transcription Translation and Splicing, Proteases & Other Enzymes

Epigenetics

GMP

GPCR

GPCR, Growth and Development, Neuronal Cell Markers, Wnt Signaling Pathway

GPCR, Neuronal Cell Markers, Neuroscience, Neurotransmission

GPCR, Neuroscience

GPCR, Signal Transduction

GPCR, Vision

Immunology

Immunology, Innate Immunity, Signal Transduction

Immunology, Signal Transduction

Immunology, Stem Cells

Immunology, Virology Bacteria and Parasites

MAP Kinase Signaling, Protein Phosphatase

metabolism

metabolism, Proteases & Other Enzymes

metabolism, Signal Transduction

Neuronal Cell Markers, Neuroscience, Neurotransmission, Vision

Neuronal Cell Markers, Neurotransmission, Protein Phosphatase

Neuroscience

Neuroscience, Signal Transduction

Neuroscience, Stem Cell Markers

Neurotransmission

Olfactory

Potassium Channels

Proteases & Other Enzymes

Proteases & Other Enzymes, Signal Transduction

Protein Kinase

Protein Phosphatase

Protein Phosphatase, Signal Transduction

Protein Turnover, Ubiquitin Proteasome Pathway

RD

Signal Transduction

Signal Transduction, Stem Cells

Stem Cells

Vision

GMP Grade

Multi-step

Protein

Research Grade