7076144
Invitrogen™ Phosphate Sensor
Phosphate Sensor
Manufacturer: Fischer Scientific
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For Use With (Equipment)
Microplate Reader
For Use With (Application)
Fluorescence Intensity
Content And Storage
Phosphate Sensor is supplied as a 500 μM solution in 10 mM Tris (pH 7.6) and 50 mM NaCl. Store product at -80°C.
Label or Dye
MDCC
Format
384-well plate
Readout
Kinetic
Target
GTPases, ATPases, Phosphatases, Phosphodiesterases
Assay
Phosphatase Assay
Detection Method
Fluorescence
Product Type
Phosphate Sensor
Excitation/Emission
430⁄450
Shipping Condition
Dry Ice
Related Products
Description
- The Phosphate Sensor is a highly sensitive reagent for the detection of inorganic phosphate, either in real-time kinetic or end-point mode
- This product is a purified form of recombinant E
- coli phosphate-binding protein labeled with the fluorophore MDCC, which is sensitive to changes in its environment
- Binding of inorganic phosphate by Phosphate Sensor is rapid and tight (Kd ∼ 0.1μM), resulting in a large increase in fluorescence
- The Phosphate Sensor detects phosphate in the high-nanomolar to low-micromolar range, which is 100-fold more sensitive than malachite green
- This reagent provides you with a sensitive assay for detecting enzymes that produce inorganic phosphate either directly or indirectly through coupled reactions, such as phosphatases, ATPases, GTPases, and phosphodiesterases.
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For Use With (Equipment): Microplate Reader
For Use With (Application): Fluorescence Intensity
Content And Storage: Phosphate Sensor is supplied as a 500 μM solution in 10 mM Tris (pH 7.6) and 50 mM NaCl. Store product at -80°C.
Label or Dye: MDCC
Format: 384-well plate
Readout: Kinetic
Target: GTPases, ATPases, Phosphatases, Phosphodiesterases
Assay: Phosphatase Assay
Detection Method: Fluorescence
Product Type: Phosphate Sensor
Excitation/Emission: 430⁄450
Shipping Condition: Dry Ice
For Use With (Equipment):
Microplate Reader
For Use With (Application):
Fluorescence Intensity
Content And Storage:
Phosphate Sensor is supplied as a 500 μM solution in 10 mM Tris (pH 7.6) and 50 mM NaCl. Store product at -80°C.
Label or Dye:
MDCC
Format:
384-well plate
Readout:
Kinetic
Target:
GTPases, ATPases, Phosphatases, Phosphodiesterases
Assay:
Phosphatase Assay
Detection Method:
Fluorescence
Product Type:
Phosphate Sensor
Excitation/Emission:
430⁄450
Shipping Condition:
Dry Ice
For Use With (Equipment): Microplate Reader, Spectrophotometer, HTS Reader
For Use With (Application): Viability Assay
Content And Storage: Store at 4°C and protect from light.
Label or Dye: __
Format: 96-well plate, Cuvettes, 384-well plate
Readout: __
Target: __
Assay: __
Detection Method: Colorimetric, Fluorescence
Product Type: Reagent
Excitation/Emission: __
Shipping Condition: Approved for shipment at Room Temperature or on Wet Ice
For Use With (Equipment):
Microplate Reader, Spectrophotometer, HTS Reader
For Use With (Application):
Viability Assay
Content And Storage:
Store at 4°C and protect from light.
Label or Dye:
__
Format:
96-well plate, Cuvettes, 384-well plate
Readout:
__
Target:
__
Assay:
__
Detection Method:
Colorimetric, Fluorescence
Product Type:
Reagent
Excitation/Emission:
__
Shipping Condition:
Approved for shipment at Room Temperature or on Wet Ice
For Use With (Equipment): __
For Use With (Application): Real Time PCR (qPCR)
Content And Storage: __
Label or Dye: __
Format: __
Readout: __
Target: __
Assay: __
Detection Method: __
Product Type: License
Excitation/Emission: __
Shipping Condition: __
For Use With (Equipment):
__
For Use With (Application):
Real Time PCR (qPCR)
Content And Storage:
__
Label or Dye:
__
Format:
__
Readout:
__
Target:
__
Assay:
__
Detection Method:
__
Product Type:
License
Excitation/Emission:
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Shipping Condition:
__
For Use With (Equipment): __
For Use With (Application): __
Content And Storage: __
Label or Dye: __
Format: __
Readout: __
Target: __
Assay: __
Detection Method: __
Product Type: Streptavidin Conjugate (fluorescent)
Excitation/Emission: __
Shipping Condition: Room Temperature
For Use With (Equipment):
__
For Use With (Application):
__
Content And Storage:
__
Label or Dye:
__
Format:
__
Readout:
__
Target:
__
Assay:
__
Detection Method:
__
Product Type:
Streptavidin Conjugate (fluorescent)
Excitation/Emission:
__
Shipping Condition:
Room Temperature