7083200

Invitrogen™ Alexa Fluor™ 488 Tyramide SuperBoost™ Kit, goat anti-rabbit IgG

Features poly-HRP-conjugated goat anti-rabbit IgG secondary antibody. Alexa Fluor™ 488 Tyramide SuperBoost™ Kit combine brightness of AlexaFluor dyes with high signal amplification of a poly-HRP-mediated tyramide labeling reaction to produce s

Manufacturer: Fischer Scientific

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Conjugate

Alexa Fluor 488

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Description

  • SuperBoost™ tyramide signal amplification is most sensitive method for detection of low abundant targets in multiplexable fluorescent immunocytochemistry (ICC), immunohistochemistry (IHC ), and in situ hybridization (ISH)
  • Features: High sensitivity for detection of low-level or hard-to-detect targets by fluorescent imaging Simple protocol and detection using standard filters Suitable for high-resolution multiplex images, co-label with DAPI, secondary antibodies, and other SuperBoost kits Requires 10-100 times less primary antibody then standard ICC/IHC/ISH experiments Benefits: Enhancement of signal using Alexa Fluor tyramides: Utilize Alexa Fluor tyramides, which react with HRP to ultimately deposit bright and photostable Alexa Fluor dye on surrounding proteins and other similar molecules Poly-HRP enhancement: Several HRP enzymes are conjugated with short polymers, enhancing signal by several fold over regular HRP systems Reaction stop solution: Include an HRP stop solution to halt the HRP reaction Reduction of background: Include blockers for elimination or reduction of endogenous peroxidase and fluorescent background signals Shelf Life: Stable for 6 months after receiving

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