Thermo Scientific™ Pierce™ Dilution-Free™ BSA Protein Standards, multichannel pipette compatible

Description

• Pierce Dilution-Free BSA Protein Standards are high-quality bovine serum albumin reference samples for generating accurate standard curves and calibration controls in total protein assays
• They are packaged as a set of seven pre-diluted standards in a multichannel pipette-friendly tubestrip, eliminating the need for serial dilutions
• The tubestrip includes a single empty tube that enables users to add their own sample buffer for the purpose of blank subtraction
• Features of Dilution-Free BSA Protein Standards include: • Multichannel pipette compatible —unique tubestrip design allows simultaneous transfer of a full standard curve dilution series to a microplate • Convenient —complete set containing seven ready-to-use dilutions • Universal —recognized as the industry standard for protein quantitation in colorimetric protein assays • Pure and stable —supplied in ultrapure 0.9% saline solution with 0.05% sodium azide • Accurate —reduce pipetting error from serial dilutions These bovine serum albumin (BSA) solutions are protein concentration reference standards for use in BCA, Bradford, and other protein assay protocols
• BSA is the universally accepted reference protein for total protein quantitation
• The set of standards is prepared from a stock solution that is calibrated by direct comparison to purified BSA (Fraction V) from the National Institute of Standards and Technology (NIST)
• Applications include: • Protein assay quantitation standard (BCA Protein Assay, Bradford Assay, etc.) • Protein recovery control for desalting and other column procedures • General calibration of spectrophotometer UV-lamp (absorbance at 280 nm) Selection of a protein standard is potentially the greatest source of error in any protein assay
• The best choice for a standard is a purified, known concentration of the most abundant protein contained in the samples being tested
• Often, a highly purified, known concentration of the protein of interest is not available or is too expensive to use as the standard or the sample itself is a mixture of many proteins (e.g., cell lysate)
• In such cases, the best standard is one that will produce a normal (i.e., average) color response curve with the selected protein assay method and is readily available to any researcher
• BSA is such a protein, and the Pierce Dilution-Free BSA Protein Standards are the most convenient source of ready-to-use BSA standard
• For greatest accuracy in estimating total protein concentration in unknown samples, it is essential to include a standard curve each time the assay is performed
• This is particularly true for the protein assay methods that produce non-linear standard curves
• Deciding on the number of standards and replicates used to define the standard curve depends upon the degree of non-linearity in the standard curve and the degree of accuracy required
• In general, fewer points are needed to construct a standard curve if the color response is linear
• Typically, standard curves are constructed using at least two replicates for each point on the curve.