Thermo Scientific™ Vivid™ CYP3A5 Green Screening Kit

Description

• Vivid™ CYP450 Screening Kits are high-throughput fluorescence-based assays for detection of enzyme-drug interactions and CYP450 inhibition
• They provide an optimized method for studying isozyme-specific CYP450-drug interactions, metabolism, and inhibition
• Vivid™ CYP450 Screening Kits offer: Easy three-step procedure, 'mix and read' format, reactions at room temperature or 37°C Superior fluorescent properties and kinetics compared to conventional fluorogenic probes High signal-to-background ratio, broad dynamic range Compatible with multiple assay formats from 96-well to 1536-well Simple Mix and Read Kit Format Vivid™ CYP450 Screening Kits include Vivid™ Substrate, Vivid™ Fluorescent Standard, reaction buffer, Vivid™ Regeneration System, NADP+, and CYP450 BACULOSOMES™ Plus Reagents
• The CYP450 BACULOSOMES™ Plus Reagents are microsomes prepared from insect cells expressing a human CYP450 isozyme (CYP3A5 in this case) and human cytochrome-P450 reductase
• CYP450 BACULOSOMES™ Plus Reagents offer a distinct advantage over human liver microsomes in that only one CYP450 isozyme is expressed, thereby preventing metabolism by other CYP450s
• Unique Vivid™ Reagents for Bright Fluorescent Signals and Low Background Vivid™ Substrates are blocked dyes that yield minimal fluorescence signal until cleaved or hydroxylated
• Oxidation at either of two potential sites releases the highly fluorescent product (Figure 1)
• They have superior fluorescence, solubility, and kinetic properties compared to conventional fluorogenic probes
• This results in higher sensitivity, greater signal-to-noise ratio, and better assay reproducibility
• Vivid™ DBOMF Substrate is the substrate included in this particular kit
• This substrate yields a product that emits a green fluorescence
• Flexible Assay Formats for Optimized Results The sensitivity of the Vivid™ CYP450 assays allows detection of weak inhibitors and miniaturization to as little as 2 μl per reaction
• Assays may be set up in kinetic mode or in end-point mode to facilitate multi-plate screening (Figure 2)
• Assays may be performed at room temperature or 37°C
• Applications High-throughput screening of enzyme-drug interactions Compound profiling for drug inhibition of cytochrome P450 isozymes Generation of predictive SAR models to guide compound acquisition