PI23246
Thermo Scientific™ Pierce™ Detergent Compatible Bradford Assay Kit
Manufacturer: Thermo Scientific™
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PI23246-Each-of-1 | In Stock | ₹ 23,828.46 |
PI23246 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 23,828.46
GST (18%): ₹ 4,289.123
Total Price: ₹ 28,117.583
Content And Storage
Detergent Compatible Bradford Assay Reagent, 450 mL, store at 4°CAlbumin Standard Ampules, 10 x 1 mL, store at room temperature
Detection Method
Colorimetric
Product Type
Protein Quantitation Assay
For Use With (Application)
Solution-based Detection, Absorbance
Quantity
300 Test Tube Assays
Assay
Bradford Assay
Product Line
Pierce™
Specificity
Not Target-Specific
For Use With (Equipment)
Spectrophotometer, Microplate Reader
Related Products
Description
- The Thermo Scientific Pierce Detergent Compatible Bradford Assay Kit is a quick and ready-to-use modification of the well-known Bradford coomassie dye-binding, colorimetric method for total protein quantitation
- Proprietary additives to the Bradford Reagent make it compatible with up to 1% or higher of detergents and lysis reagents that are commonly used in life science research, including Triton™ X-100 and NP-40
- Convenient - detergent-free standard curve Flexible - compatible with samples both with and without detergent Minimal sample - requires only 10μL for microplate procedure Colorimetric - measure with a standard spectrophotometer or plate reader (595 nm) Easy to use - single reagent, no working reagent preparation required Fast - 10 minute incubation at room temperature Broad range - detects protein concentration in the ranges of 2 to 1500μg/mL Similar to the Bradford method, coomassie dye binds protein in an acidic medium causing an immediate shift in absorption maximum from 465 nm to 595 nm with a concomitant color change from green to blue
- In addition, the assay is complete in just 10 minutes
- The protein assay can be performed in either test tube or microplate format
- The standard working range is 100-1500μg/mL with up to 1% detergent (or higher in some cases)
- Protein concentrations are estimated by reference to absorbances obtained for a series of standard protein dilutions, typically bovine serum albumin (BSA), which are assayed alongside the unknown samples
- Because the color response with coomassie dye is non-linear with increasing protein concentration, a standard curve must be completed with each assay
- Standards can be used directly without preparing them in the same detergent found in the test samples.
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Content And Storage: Detergent Compatible Bradford Assay Reagent, 450 mL, store at 4°CAlbumin Standard Ampules, 10 x 1 mL, store at room temperature
Detection Method: Colorimetric
Product Type: Protein Quantitation Assay
For Use With (Application): Solution-based Detection, Absorbance
Quantity: 300 Test Tube Assays
Assay: Bradford Assay
Product Line: Pierce™
Specificity: Not Target-Specific
For Use With (Equipment): Spectrophotometer, Microplate Reader
Content And Storage:
Detergent Compatible Bradford Assay Reagent, 450 mL, store at 4°CAlbumin Standard Ampules, 10 x 1 mL, store at room temperature
Detection Method:
Colorimetric
Product Type:
Protein Quantitation Assay
For Use With (Application):
Solution-based Detection, Absorbance
Quantity:
300 Test Tube Assays
Assay:
Bradford Assay
Product Line:
Pierce™
Specificity:
Not Target-Specific
For Use With (Equipment):
Spectrophotometer, Microplate Reader
Content And Storage: Sufficient For: 250 tube assays with 25 μL samples• BCA Reagent A, 250 mL• BCA Reagent B, 25 mL• Compatibility Reagent, 10 x 20 mg• Reconstitution Buffer, 15 mL• Albumin Standard Ampules, 2mg/mL, 10 x 1 mLStore at room temperature
Detection Method: Colorimetric
Product Type: Protein Quantitation Assay
For Use With (Application): Solution-based Detection, Absorbance
Quantity: 275 mL
Assay: BCA Assay
Product Line: Pierce™
Specificity: Not Target-Specific
For Use With (Equipment): Spectrophotometer, Microplate Reader
Content And Storage:
Sufficient For: 250 tube assays with 25 μL samples• BCA Reagent A, 250 mL• BCA Reagent B, 25 mL• Compatibility Reagent, 10 x 20 mg• Reconstitution Buffer, 15 mL• Albumin Standard Ampules, 2mg/mL, 10 x 1 mLStore at room temperature
Detection Method:
Colorimetric
Product Type:
Protein Quantitation Assay
For Use With (Application):
Solution-based Detection, Absorbance
Quantity:
275 mL
Assay:
BCA Assay
Product Line:
Pierce™
Specificity:
Not Target-Specific
For Use With (Equipment):
Spectrophotometer, Microplate Reader
Content And Storage: Sufficient For: 980 microplate assays with 9 μL samples• BCA Reagent A, 250 mL• BCA Reagent B, 25 mL• Compatibility Reagent, 48 x 10 mg• Reconstitution Buffer, 15 mL• Albumin Standard Ampules, 2mg/mL, 10 x 1 mL• 96-Well Microplates, 20 platesStore at room temperature
Detection Method: Colorimetric
Product Type: Protein Quantitation Assay
For Use With (Application): Solution-based Detection, Absorbance
Quantity: 275 mL
Assay: BCA Assay
Product Line: Pierce™
Specificity: Not Target-Specific
For Use With (Equipment): Spectrophotometer, Microplate Reader
Content And Storage:
Sufficient For: 980 microplate assays with 9 μL samples• BCA Reagent A, 250 mL• BCA Reagent B, 25 mL• Compatibility Reagent, 48 x 10 mg• Reconstitution Buffer, 15 mL• Albumin Standard Ampules, 2mg/mL, 10 x 1 mL• 96-Well Microplates, 20 platesStore at room temperature
Detection Method:
Colorimetric
Product Type:
Protein Quantitation Assay
For Use With (Application):
Solution-based Detection, Absorbance
Quantity:
275 mL
Assay:
BCA Assay
Product Line:
Pierce™
Specificity:
Not Target-Specific
For Use With (Equipment):
Spectrophotometer, Microplate Reader
Content And Storage: Sufficient For: Preparing standard curves for glycoprotein carbohydrate estimation• Lysozyme, lyophilized, 2.5 mg• BSA, lyophilized, 2.5 mg• Ovalbumin, lyophilized, 2.5 mg• Apo-tranferrin, lyophilized, 2.5 mg• Fetuin, lyophilized, 0.25 mg• alpha-Acid Glycoprotein, 0.25 mg
Detection Method: Colorimetric
Product Type: Glycoprotein Carbohydrate Estimation
For Use With (Application): Internal Standard
Quantity: 5 Protein
Assay: Glycoprotein Standards
Product Line: Pierce™
Specificity: Not Target-Specific
For Use With (Equipment): Spectrophotometer, Microplate Reader
Content And Storage:
Sufficient For: Preparing standard curves for glycoprotein carbohydrate estimation• Lysozyme, lyophilized, 2.5 mg• BSA, lyophilized, 2.5 mg• Ovalbumin, lyophilized, 2.5 mg• Apo-tranferrin, lyophilized, 2.5 mg• Fetuin, lyophilized, 0.25 mg• alpha-Acid Glycoprotein, 0.25 mg
Detection Method:
Colorimetric
Product Type:
Glycoprotein Carbohydrate Estimation
For Use With (Application):
Internal Standard
Quantity:
5 Protein
Assay:
Glycoprotein Standards
Product Line:
Pierce™
Specificity:
Not Target-Specific
For Use With (Equipment):
Spectrophotometer, Microplate Reader