PI23270
Thermo Scientific™ Pierce™ Phosphoprotein Phosphate Estimation Assay Kit
Manufacturer: Thermo Scientific™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PI23270-Each-of-1 | In Stock | ₹ 57,753.00 |
PI23270 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 57,753.00
GST (18%): ₹ 10,395.54
Total Price: ₹ 68,148.54
Content And Storage
Sufficient For: 500 tube assays or 1920 microplate assays• Ammonium molybdate Solution, 25 mL• Malachite Green Solution, 75 mL• Phosvitin Positive Control, 1 mg• TBS Pack (makes 500 mL), 1 packUpon receipt, store components at room temperature. Once dissolved, store Phosvitin solution at 4°C (or -20°C for long-term storage).
Detection Method
Colorimetric
Product Type
Phosphoprotein Phosphate Estimation
Sufficient For
500 Tube Assays or 1920 Microplate Assays
For Use With (Equipment)
Spectrophotometer, Microplate Reader
Assay
Phosphoprotein Phosphate Estimation Assay
Product Line
Pierce™
Specificity
Not Target-Specific
For Use With (Application)
Solution-based Detection, Absorbance
Quantity
100 mL
Related Products
Description
- Thermo Scientific™ Phosphoprotein Phosphate Estimation Kit enables classification and identification of proteins as phosphorylated (serine and threonine), as well as semi-quantitative assessment of the phosphorylation level
- The Phosphoprotein Phosphate Estimation Assay Kit is designed to aid in characterization of the status and extent of phosphorylation of purified protein samples
- The assay is based on the alkaline hydrolysis of phosphate from seryl and threonyl residues in phosphoproteins and quantification of the released phosphate with malachite green and ammonium molybdate
- The assay is easily performed in 96-well microplates or test tubes and is completed in about one hour
- The assay can be used to identify whether a purified protein contains either phospho-serine (p-Ser) or phospho-threonine (p-Thr) as well as to estimate the level of this type of phosphorylation
- For quantitation, the test protein sample is compared with specific concentrations of phosvitin, a phosphoprotein of known phosphorylation level
- The alkaline hydrolysis step does not release phosphate from phospho-tyrosine (p-Tyr) residues in peptide linkage
- Therefore, a negative result for an unknown purified protein preparation indicates that the protein is either (1) not a phosphoprotein or (2) is phosphorylated exclusively at tyrosine residues
- In the latter case, Western blot analysis using an anti-phosphotyrosine antibody will be necessary to distinguish between these two possibilities
- Highlights: Specific – measures phosphoserine (p-Ser) and phosphothreonine (p-Thr) only; does not measure phosphotyrosine (p-Tyr) Convenient – test tube and 96-well microplate protocols included and require less than 90 minutes to perform Semi-quantitative – calculate moles of phosphate (P) per mole of protein using the included phosvitin standard Customizable – format is adaptable to development of specific assays for pure phosphoproteins when previously characterized standards are available Includes: Ammonium molybdate solution, malachite green solution, Tris-buffered saline pack and phopvitin positive control Recommended for: Identify proteins as containing phosphoserine or phosphothreonine phosphorylations; Estimate the amount of pS and pT phosphorylation; Develop specific quantitative assays for well-characterized phosphoproteins of interest
Compare Similar Items
Show Difference
Content And Storage: Sufficient For: 500 tube assays or 1920 microplate assays• Ammonium molybdate Solution, 25 mL• Malachite Green Solution, 75 mL• Phosvitin Positive Control, 1 mg• TBS Pack (makes 500 mL), 1 packUpon receipt, store components at room temperature. Once dissolved, store Phosvitin solution at 4°C (or -20°C for long-term storage).
Detection Method: Colorimetric
Product Type: Phosphoprotein Phosphate Estimation
Sufficient For: 500 Tube Assays or 1920 Microplate Assays
For Use With (Equipment): Spectrophotometer, Microplate Reader
Assay: Phosphoprotein Phosphate Estimation Assay
Product Line: Pierce™
Specificity: Not Target-Specific
For Use With (Application): Solution-based Detection, Absorbance
Quantity: 100 mL
Content And Storage:
Sufficient For: 500 tube assays or 1920 microplate assays• Ammonium molybdate Solution, 25 mL• Malachite Green Solution, 75 mL• Phosvitin Positive Control, 1 mg• TBS Pack (makes 500 mL), 1 packUpon receipt, store components at room temperature. Once dissolved, store Phosvitin solution at 4°C (or -20°C for long-term storage).
Detection Method:
Colorimetric
Product Type:
Phosphoprotein Phosphate Estimation
Sufficient For:
500 Tube Assays or 1920 Microplate Assays
For Use With (Equipment):
Spectrophotometer, Microplate Reader
Assay:
Phosphoprotein Phosphate Estimation Assay
Product Line:
Pierce™
Specificity:
Not Target-Specific
For Use With (Application):
Solution-based Detection, Absorbance
Quantity:
100 mL
Content And Storage: Colorimettric Assay Reagent A, 50 mLColorimetric Assay Reagent B, 2 x 25 mLColorimetric Assay Reagent C, 2 mLPeptide Digest Assay Standard, 1.5 mLStore in refrigerator (2–8°C).
Detection Method: Colorimetric
Product Type: Peptide Assay
Sufficient For: __
For Use With (Equipment): Microplate Reader
Assay: __
Product Line: Pierce™
Specificity: __
For Use With (Application): __
Quantity: 500 Assays
Content And Storage:
Colorimettric Assay Reagent A, 50 mLColorimetric Assay Reagent B, 2 x 25 mLColorimetric Assay Reagent C, 2 mLPeptide Digest Assay Standard, 1.5 mLStore in refrigerator (2–8°C).
Detection Method:
Colorimetric
Product Type:
Peptide Assay
Sufficient For:
__
For Use With (Equipment):
Microplate Reader
Assay:
__
Product Line:
Pierce™
Specificity:
__
For Use With (Application):
__
Quantity:
500 Assays
Content And Storage: Sufficient For: 100 tube assays or 500 microplate assays with non-lipid samples• Reagent A, 1 mL• Reagent B, 2 x 50 mLStore at 4°C.
Detection Method: Colorimetric
Product Type: Quantitative Peroxide Assay
Sufficient For: 100 Tube Assays or 500 Microplate Assays
For Use With (Equipment): Spectrophotometer, Microplate Reader
Assay: Quantitative Peroxide Assay
Product Line: Pierce™
Specificity: Not Target-Specific
For Use With (Application): Solution-based Detection, Absorbance
Quantity: __
Content And Storage:
Sufficient For: 100 tube assays or 500 microplate assays with non-lipid samples• Reagent A, 1 mL• Reagent B, 2 x 50 mLStore at 4°C.
Detection Method:
Colorimetric
Product Type:
Quantitative Peroxide Assay
Sufficient For:
100 Tube Assays or 500 Microplate Assays
For Use With (Equipment):
Spectrophotometer, Microplate Reader
Assay:
Quantitative Peroxide Assay
Product Line:
Pierce™
Specificity:
Not Target-Specific
For Use With (Application):
Solution-based Detection, Absorbance
Quantity:
__
Content And Storage: Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: 2 to 8°C.
Detection Method: Colorimetric
Product Type: Quantitative Peroxide Assay
Sufficient For: __
For Use With (Equipment): Spectrophotometer, Microplate Reader
Assay: Peroxide Assay Reagent A
Product Line: Pierce™
Specificity: Not Target-Specific
For Use With (Application): Solution-based Detection, Absorbance
Quantity: __
Content And Storage:
Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: 2 to 8°C.
Detection Method:
Colorimetric
Product Type:
Quantitative Peroxide Assay
Sufficient For:
__
For Use With (Equipment):
Spectrophotometer, Microplate Reader
Assay:
Peroxide Assay Reagent A
Product Line:
Pierce™
Specificity:
Not Target-Specific
For Use With (Application):
Solution-based Detection, Absorbance
Quantity:
__