Thermo Scientific™ pEF1α Tluc16-DD Vector for Luciferase Assays

Description

• The Thermo Scientific pMCS Tluc16 Vectors are multiple cloning site (MCS) vectors designed to accept a promoter sequence for study of gene regulation using the intracellular TurboLuc™16 (Tluc16) luciferase reporter
• Intracellular Tluc16 luciferase gene, optimized for high expression in mammalian systems Multiple cloning sites provide versatility for transfer of regulatory elements from one vector to another Synthetic polyA terminator and Transcriptional Pause Site (TPS) included upstream of MCS to minimize non-specific transcriptional read-through Dual-destabilization (DD) technology reduces accumulation of Tluc16 luciferase mRNA and protein in cells, enhancing the responsiveness of the assay Optimized minimal core promoter (minP) and 5' UTR region for efficient expression of Tluc16 luciferase Hygromycin resistance gene for use as selection marker to generate mammalian stable cell lines Prepared pNF-κB, and pCRE Tluc16-DD Vectors are designed to monitor the activation of NFκB protein and cAMP-binding protein (CREB) in mammalian cells Prepared pEF1α variety includes a modified version of the Elongated Factor 1α (EF1α) promoter for constitutive Tluc16 luciferase expression in mammalian cells Tluc16 luciferase is a 16 kDa, novel, intracellular luciferase derived from the marine copedod Metridia luciferase family
• The wild-type luciferase has been modified to reduce its size, increase its brightness, and enable its intracellular expression
• A synthetic polyA terminator and a Transcriptional Pause Site (TPS) are included upstream of the MCS to minimize non-specific transcriptional read-through
• The Tluc16 luciferase activity can be measured using the Thermo Scientific TurboLuc™ One-Step Glow Assay Kit.