Vectors That Express Both Firefly and NanoLuc(R) Luciferases from the Same Transcript Firefly and NanoLuc(R) luciferase have dissimilar compound interference profiles for better identifying more false-positives versus individually Using two different transcriptional reporters reduces false hit rates and increases the identification of true biological hits luc2 and NlucP provide a bright reporter combination compatible with low-copy-number and plate scale-up, and provide greater signal-to-background ratios