Thermo Scientific Chemicals RIPA buffer (5X)

Description

• RIPA buffer is used to lyse cells and tissues
• It is used to lyse cultured mammalian cells from both plated cells and cells pelleted from suspension cultures
• This buffer enables protein extraction from cytoplasmic, membrane and and is particularly useful for disruption of nuclear membranes in the preparation of nuclear extracts
• It is compatible with many applications, including reporter assays, protein assays, immunoassays and protein purification. This product is ideal for general cell-tissue lysis and sub cellular organelle or molecular fractionation, including immunoprecipation
• This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio
• Some documentation and label information may refer to the legacy brand
• The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals
• General Description • RIPA can be used for isolation of cytoplasmic, membrane, nuclear, and mitochondrial proteins • Protease inhibitors can be added to RIPA buffer to assist in protein isolation Application • RIPA buffer is suitable for use in protein isolation from culture cells or homogenized tissues • Downstream applications of RIPA buffer include ELISA, western blot, immunoprecipitation, and SDS-PAGE