Thermo Scientific™ T-PER™ Tissue Protein Extraction Reagent

Description

• Perform mild extraction of total protein from tissue samples with Thermo Scientific™ T-PER Tissue Protein Extraction Reagent
• This tissue cell lysis reagent utilizes a proprietary detergent in 25mM bicine and 150mM sodium chloride (pH 7.6) to maximize the efficiency of protein solubilization from mammalian tissue samples by homogenization
• The simple composition is compatible with additives such as protease inhibitors, salts, reducing agents and chelating agents, providing versatility for many different sample types and lysis applications
• Cell lysates prepared with T-PER Reagent are directly compatible with reporter assays (e.g., luciferase, beta-galactosidase, chloramphenicol acetyl transferase), protein kinase assays (e.g., PKA, PKC, tyrosine kinase), immunoassays (e.g., Western blots, ELISAs, RIAs) and/or protein purification procedures
• Highlights: Simple procedure – just homogenize tissue sample in 1:20 (w/v) of tissue to T-PER Reagent, then centrifuge to pellet cell/tissue debris Mild and convenient – detergent is dialyzable for quick and easy removal; can be used with additional components (e.g., protease inhibitors, salts, reducing agents, chelating agents, etc.) Flexible – lysate may be used for reporter assays, protein kinase assays, immunoassays and/or protein purifications Compatible – the lysate is compatible with standard protein assays such as Thermo Scientific Pierce™ Coomassie Plus™ (Bradford) Protein Assay