PI90084
Thermo Scientific™ B-PER™ Bacterial Protein Extraction Reagent
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PI90084-Each-of-1 | In Stock | ₹ 33,368.40 |
PI90084 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 33,368.40
GST (18%): ₹ 6,006.312
Total Price: ₹ 39,374.712
Description
B-PER Bacterial Protein Extraction Reagent
Content And Storage
Store B-PER Reagent at ambient temperature. Store Lysozyme and DNaseI at -20°C.
Product Type
Bacterial Protein Extraction Reagent
Product Line
B-PER™
Quantity
250 mL
Volume (Metric)
250 mL
Related Products
Description
- Perform efficient, gentle lysis and extraction of E.coli and other bacterial cells with Thermo Scientific™ B-PER Bacterial Protein Extraction Reagents
- These easy-to-use cell lysis reagents are nonionic detergent solutions that effectively disrupt cells and solubilize native or recombinant proteins without denaturation
- Certain formulations include DNase I and lysozyme enzyme supplements to improve yield of large molecular weight proteins that are difficult to purify
- B-PER Reagents are compatible with the addition of standard protease inhibitors and with downstream protein purification methods, such as GST purification and His-tagged immobilized metal affinity chromatography (IMAC)
- Highlights: Ready to use – B-PER Reagents provide one-step E.coli cell lysis by a mild, nonionic detergent (proprietary) in your choice of Tris or phosphate buffer formulations Fast and simple – just add B-PER Reagent to a bacterial pellet, shake for 10 minutes and recover soluble proteins after pelleting the cell debris Excellent yields – recover both soluble and insoluble recombinant protein from bacterial lysates and purify inclusion bodies to near-homogeneous levels Flexible – B-PER Reagents are suitable for any scale of protein extraction and are available in phosphate and 1X and 2X Tris formulations, with and without enzymes Compatible – completely compatible with addition of protease inhibitor cocktails, and resulting protein extract can be used in protein assays, typical affinity purification methods (e.g., GST, 6xHis) and other applications Convenient, ready-to-use formats: B-PER and B-PER II (2X) Reagents are free of enzymatic components B-PER Reagent (in phosphate buffer) is an amine-free formulation for direct compatibility with labeling and crosslinking B-PER with Enzymes and B-PER Direct with Enzymes include lysozyme and DNase I for improved lysis and recovery Includes: Reagent packages are available in Tris and phosphate buffer formulations; Kits contain Tris-based B-PER Reagent plus lysozyme and DNase I enzymes.
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Description: B-PER Bacterial Protein Extraction Reagent
Content And Storage: Store B-PER Reagent at ambient temperature. Store Lysozyme and DNaseI at -20°C.
Product Type: Bacterial Protein Extraction Reagent
Product Line: B-PER™
Quantity: 250 mL
Volume (Metric): 250 mL
Description:
B-PER Bacterial Protein Extraction Reagent
Content And Storage:
Store B-PER Reagent at ambient temperature. Store Lysozyme and DNaseI at -20°C.
Product Type:
Bacterial Protein Extraction Reagent
Product Line:
B-PER™
Quantity:
250 mL
Volume (Metric):
250 mL
Description: HisPur Cobalt Spin Purification Kit, 0.2mL
Content And Storage: Sufficient For: Purifying ∼2 mg of His-tagged protein per column• HisPur Cobalt Spin Columns, 0.2 mL, 25 columns• Equilibration/Wash Buffer, 100 mL• Elution Buffer, 50 mL• Collection Tubes, 80 tubesStore at 4°C.
Product Type: __
Product Line: HisPur™
Quantity: 25 Column Kit
Volume (Metric): __
Description:
HisPur Cobalt Spin Purification Kit, 0.2mL
Content And Storage:
Sufficient For: Purifying ∼2 mg of His-tagged protein per column• HisPur Cobalt Spin Columns, 0.2 mL, 25 columns• Equilibration/Wash Buffer, 100 mL• Elution Buffer, 50 mL• Collection Tubes, 80 tubesStore at 4°C.
Product Type:
__
Product Line:
HisPur™
Quantity:
25 Column Kit
Volume (Metric):
__
Description: HisPur Cobalt Spin Purification Kit, 1mL
Content And Storage: Sufficient For: Purifying ∼10 mg of His-tagged protein per column• HisPur Cobalt Spin Columns, 1 mL, 5 columns• Equilibration/Wash Buffer, 100 mL• Elution Buffer, 50 mLStore at 4°C.
Product Type: __
Product Line: HisPur™
Quantity: 5 columns
Volume (Metric): __
Description:
HisPur Cobalt Spin Purification Kit, 1mL
Content And Storage:
Sufficient For: Purifying ∼10 mg of His-tagged protein per column• HisPur Cobalt Spin Columns, 1 mL, 5 columns• Equilibration/Wash Buffer, 100 mL• Elution Buffer, 50 mLStore at 4°C.
Product Type:
__
Product Line:
HisPur™
Quantity:
5 columns
Volume (Metric):
__
Description: HisPur Cobalt Spin Purification Kit, 3mL
Content And Storage: Sufficient For: Purifying ∼30 mg of His-tagged protein per column• HisPur Cobalt Spin Columns, 3 mL, 5 columns• Equilibration/Wash Buffer, 225 mL• Elution Buffer, 75 mLStore at 4°C.
Product Type: __
Product Line: HisPur™
Quantity: 5 Column Kit
Volume (Metric): __
Description:
HisPur Cobalt Spin Purification Kit, 3mL
Content And Storage:
Sufficient For: Purifying ∼30 mg of His-tagged protein per column• HisPur Cobalt Spin Columns, 3 mL, 5 columns• Equilibration/Wash Buffer, 225 mL• Elution Buffer, 75 mLStore at 4°C.
Product Type:
__
Product Line:
HisPur™
Quantity:
5 Column Kit
Volume (Metric):
__