Thermo Scientific™ SureQuant™ IP-MS Sample Preparation Kits

Description

• Thermo Scientific SureQuant IP-MS Sample Preparation kits (Protein A/G or streptavidin) provide an optimized solution for the immunoprecipitation and MS peptide preparation of samples upstream of LC-MS/MS analysis
• These kits have been rigorously verified using numerous multiplexed target antigens with varying expression levels
• The SureQuant product family enables the use of the immunoprecipitation (IP) sample prep kits and peptide quantitation modules together or independently for SRM/PRM analysis
• • Complete —kits include reagents for successful immunoprecipitation and MS peptide preparation for target proteins for SRM/PRM analysis • Multiplex —IP-MS kits have been designed for multiplex IP using both Protein A/G and Streptavidin magnetic beads • Flexible —universal reagents enable individualized selection of targets for IP to be used alone or combined with SureQuant peptide quantitation panels • Optimized —simplified and condensed procedure to prepare samples for LC-MS analysis • Low background —binding, wash, and elution buffers optimized to remove contaminants and minimize enrichment of background proteins The SureQuant Protein A/G and Streptavidin IP-MS Sample Preparation kits have been designed to support targeted quantitation using SureQuant or SRM/PRM analysis
• The kits contain high-quality Pierce Protein A/G or Streptavidin magnetic beads together with reagents that have been optimized for MS compatibility
• The streamlined procedure enables digestion immediately after the immunoprecipitation elution step, facilitating MS sample preparation in ∼4 hours for same-day LC-MS/MS analysis
• Applications: • Enrichment of low abundance targets to improve MS sensitivity • Identification of target proteins that are not detectable by western blotting • Quantitation of target proteins by LC-MS analysis • Screening of antibodies for IP-MS • Enrichment of target proteins upstream of targeted analysis (SRM)