Thermo Scientific™ EasyPep™ Magnetic MS Sample Preparation Kits and Proteomics Magnetic Clean-up Beads

Description

• Proteomic sample preparation for MS analysis is complex, resulting in challenges with reproducibility and peptide quality
• For MS sample preparation methods that utilize magnetic beads, a major challenge is the compatibility of the beads with the harsh conditions required for sample processing
• When exposed to extreme pH or solvents, traditional magnetic beads often clump or dissolve, resulting in increased variability
• Pierce Proteomics Magnetic Clean-up Beads have been designed for compatibility with common solvents (e.g
• acetonitrile, ethanol, isopropyl alcohol, and acetone) and wide range of pH (e.g
• pH 2-13) to enable fast, efficient, and reproducible removal of a wide variety of detergents, salts, and other mass spectrometry (MS)-incompatible contaminants from protein and peptide samples
• The beads can be successfully used to clean up proteins derived from different sample types (e.g
• purified proteins, cell lysates, tissue extracts, and biological fluids) and protein input amounts (e.g
• 1 µg to 1 mg) using manual magnetic processing or automated workflows
• EasyPep Magnetic MS Sample Prep Kits integrate optimized EasyPep sample preparation chemistry with the Proteomics Magnetic Clean-up Beads to support manual and automated magnetic bead sample processing, including the King Fisher Instrument Series
• The complete kits contain pre-formulated buffers, MS-grade enzyme mix, magnetic beads for peptide clean-up, and an optimized protocol to generate MS-ready peptide samples in less than 3 hours
• The kits are suitable for processing protein samples from 15–100 µg with a high yield of detergent-free peptides ready for direct LC-MS analysis without the need to SpeedVac dry and reconstitute before injection.  In addition, the peptide samples are compatible with isobaric tag (e.g., TMT/TMTpro Reagents) labeling and high pH reversed-phase fractionation.