Thermo Scientific™ Pierce™ High pH Reversed-Phase Peptide Fractionation Kit

Description

• In order to enable deep proteome sequencing, it is often necessary to reduce the sample complexity by fractionation in an orthogonal dimension prior to LC/MS analysis
• The Pierce High pH Reversed-Phase Peptide Fractionation Kit utilizes high pH reversed-phase chromatography to separate peptides by hydrophobicity, and provides excellent orthogonality to low pH reversed-phase LC-MS gradients
• The kit has been designed to improve protein identification through the use of a proprietary reversed-phase resin in an easy-to-use spin column format with a high-pH fractionation protocol
• Thermo Scientific Pierce High pH Reversed-Phase Peptide Fractionation Kit increases protein identification from LC/MS analysis through orthogonal peptide fractionation of complex peptide samples
• Easy-to-use - resin provided in single-use spin column format Improved protein identifications - protein identifications increased by ≥50% when compared to unfractionated samples Reproducible - elution profiles and fractional resolution vary by less than 20% Optimized - robust procedure for maximal protein identification and peptide recovery while minimizing fractional overlap Compatible - reagents validated with a variety of complex samples, including TMT™-labeled peptides In order to enable deep proteome sequencing, it is often necessary to reduce the sample complexity by fractionation in an orthogonal dimension prior to LC/MS analysis
• The Pierce High pH Reversed-Phase Peptide Fractionation Kit utilizes high pH reversed-phase chromatography to separate peptides by hydrophobicity and provides excellent orthogonality to low pH reversed-phase LC-MS gradients
• The kit has been designed to improve protein identification through the use of a proprietary reversed-phase resin in an easy-to-use spin column format with a high-pH fractionation protocol
• In contrast to strong cation exchange (SCX) fractionation, the high-pH reversed-phase fractions do not require an additional desalting step before LC/MS analysis
• The High pH Reversed-phase Peptide Fractionation Kit includes a high pH buffer (0.1% triethylamine) and twelve spin columns containing pH-resistant reversed-phase resin
• Each reversed-phase fractionation spin column enables fractionation of 10 to 100μg of peptide sample using a microcentrifuge
• Native, phosphorylated, Tandem Mass Tag™ (TMT)-labeled, and other complex peptide mixture samples can be fractionated using this kit
• Combining the search results generated by the individual fractions helps improve protein sequence coverage and increases the number of identified proteins relative to unfractionated samples
• Applications: Reducing sample complexity to identify targets of interest Conducting systems biology studies Reducing sample complexity to improve quantitation studies