Thermo Scientific™ Pierce™ HeLa Protein Digest Standard

Description

• Use the Thermo Scientific™ Pierce™ HeLa Protein Digest Standard, a highly validated mammalian protein digest, as a quality control sample for mass spectrometry (MS) analysis of complex proteomic samples
• The Thermo Scientific Pierce HeLa Protein Digest Standard is a highly validated mammalian protein digest that may be used as a quality control sample for mass spectrometry (MS) analysis of complex proteomic samples
• The protein digest standard is derived from a well-established adenocarcinoma reference HeLa S3 cell line
• HeLa S3 cells express over 15,000 proteins with relevant post-translational modifications, making this cell line an ideal standard for complex proteome mass spectrometry applications
• The cell lysate has been digested with both LysC and trypsin to minimize tryptic missed cleavages and improve protein sequence coverage
• Moreover, unlike other commercially available protein digests for MS, the Pierce HeLa Protein Digest Standard meets stringent quality testing specifications, including peptide quality, digestion efficiency, and lot-to-lot digest uniformity
• Highlights: Positive control sample – complex mammalian proteome sample protein digest (>15,000 proteins) from the HeLa S3 cell line Thorough tryptic digestion – prepared using both LysC and trypsin to ensure less than 10% tryptic missed cleavages Validated peptide quality – less than 10% methionine oxidation and less than 10% lysine carbamylation Rigorously tested – high-quality, consistent protein digest guaranteed and documented via lot-specific certificates of analysis Stable – provided in a stable lyophilized format Applications: Qualitative LC assessment LC/MS standardization LC/MS method development TMT™ Reagent method development Product Details: The Pierce HeLa Protein Digest Standard is a lyophilized tryptic peptide mixture that can be used as a quality control standard for liquid chromatography (LC) separation, MS method development and MS performance benchmarking
• The digest is specifically formulated for LC/MS experiments and does not contain salts or detergents
• Using the digest standard routinely before analysis of complex samples makes it possible to monitor and normalize LC/MS performance between samples and over time.