Thermo Scientific™ High Select™ Depletion Spin Columns

Description

• Analysis of human fluids is often complicated by the presence of high concentrations of serum proteins, including albumin and IgG that can make up more than 70% of total serum protein
• Removal of albumin, immunoglobulins, and other abundant proteins is essential for the study of low-abundance proteins
• Thermo Scientific High Select HSA/Immunoglobulin and Top 14 Abundant Protein depletion spin columns and resins enable depletion of the most abundant proteins in human serum and plasma for improved detection of low-abundance proteins using mass spectrometry analysis or 1D or 2D gel electrophoresis
• • Flexible —resin provided in mini and midi column formats as well as bulk for custom filling options • Optimized —removes >95% of IgG and >95% of albumin (HSA/Immunoglobulin); removes >95% of IgG and >95% of albumin, plus 12 other abundant proteins (Top 14) • Fast —process samples in ∼10 minutes • Economical —cost-effective spin columns are priced for single use • Reduced carry-over —one-time use prevents protein carryover and experimental variability High Select HSA/Immunoglobulin Depletion Resin uses highly specific, immobilized anti-HSA and anti-immuoglobulin antibodies (IgG, IgA, IgM, IgD, and IgE) to remove human serum albumin (HSA) and all major subclasses of immunoglobulins from serum and plasma
• High Select Top14 Abundant Protein Depletion Resin uses highly specific immobilized antibodies to remove human serum albumin (HSA), albumin, IgG, IgA, IgM, IgD, IgE, kappa and lambda light chains, alpha-1-acidglycoprotein, alpha-1-antitrypsin, alpha-2-macroglobulin, apolipoprotein A1, fibrinogen, haptoglobin, and transferrin from serum, plasma, or spinal fluids
• The resins are provided in an economical and convenient spin column format specifically designed for one-step processing and single use or bulk format for custom filling.