Thermo Scientific™ AccelerOme™ TMT11plex MS Sample Preparation Kits

Description

• The AccelerOme TMT11plex MS Sample Preparation Kit integrates pre-formulated buffers, MS-grade trypsin/Lys-C enzyme mix, and peptide clean-up cartridges with TMT11plex label reagents for reproducible sample preparation of TMT-labeled peptides
• Features of the TMT11plex MS Sample Preparation Kit include: • Optimized —EasyPep kit chemistry combined with TMT11plex labeling reagents specifically designed for use with the AccelerOme Automated Sample Prep System • Convenient —complete set of ready-to-use proteomic sample prep, clean-up, and TMT-labeling reagents for easy loading onto the instrument • Robust —reagents combined with AccelerOme ecosystem provide reproducible, high-quality TMT-labeled peptides with greater than 95% labeling efficiency • Flexible —sample prep and TMT-labeling reagents verified on system using cells, plasma, proteins, and tissue samples for 10–100 μg of sample input • Multiplex —enables concurrent mass spectrometry analysis of up to 11 samples The AccelerOme TMT11plex MS Sample Preparation Kit integrates pre-formulated buffers, MS-grade trypsin/Lys-C enzyme mix, and peptide clean-up cartridges with TMT11plex label reagents for reproducible sample preparation of TMT-labeled peptides
• These single-use components are conveniently provided in labeled trays for direct placement on the AccelerOme system
• Additionally, a peptide retention time calibration standard is also included with the kit that can be spiked into the sample before LC-MS analysis to assess system performance
• This kit provides a complete reagent solution for automated sample preparation of TMT11plex labeled samples to produce high quality, mass spectrometry ready peptides
• The AccelerOme reagent portfolio is an integral part of the AccelerOme Automated system solution to provide robust proteomic mass spec sample preparation
• The instrument hardware is built on a proven liquid handling format for reproducible sample processing and optimized on-line μSPE sample clean-up for excellent peptide recovery
• TMT label reconstitution and sample labeling and pooling have been incorporated before sample clean-up
• Peptide quantitation post-clean-up is achieved through accurate UV spectrophotometric analysis to determine peptide concentrations prior to LC-MS analysis
• The complete system (kits + reagents + software) was designed to provide high quality peptides while improving productivity with an easy-to-use automated system
• When combined with the industry-leading, high-resolution Orbitrap instruments and software, TMT11plex reagents provide integrated total solutions for quantitative protein expression analysis by mass spectrometry
• These products are subject to a limited use label license.