Thermo Scientific™ Accessing the Phosphoproteome Bundle

Description

• High-Select Fe-NTA Phosphopeptide Enrichment Kit enriches phosphopeptides from complex samples using iron-chelate resin in spin columns
• Each column can enrich phosphopeptides from 0.5 to 5 mg of total protein digest
• Together with pre-formulated buffers, the improved and simplified procedure enriches up to 150µg of phosphopeptides with greater than 90% selectivity in less than 45 minutes High-Select TiO2 enrichment kit includes 24 titanium dioxide (TiO2) spin tips with optimized buffers to facilitate preparation of enriched phosphopeptides
• Each column can enrich phosphopeptides from 0.5 to 3mg of total protein digest as starting sample
• The optimized buffers and simplified procedure eliminate the need for pyrrolidine in the elution buffer and subsequent clean-up using graphite spin columns
• Use of this kit results in enrichment of up to 150 µg of phosphopeptides with greater than 90% selectivity The High-Select TiO2 Phosphopeptide Enrichment Kit is complementary to the High-Select Fe-NTA Phosphopeptide Enrichment Kit, with both kits enriching similar numbers of phosphopeptides but different subpopulations of phosphorylated peptides