Vivapure maxiprep Spin columns are pre-packed centrifugal devices for purification of monoclonal and polyclonal antibodies from serum, ascites and cell culture supernatants
The rapid 1-2-3 bind-wash-elute protocol especially lends itself to screening applications, where many samples are processed in parallel
Vivapure maxiprep Spin columns can conveniently be used in a centrifuge with swing-out rotor for standard 50 ml tubes
Binding capacity is about 20 mg IgG per unit
The low hold-up volume ensures high solute recovery with minimal loss by non-specific adsorption
Vivapure maxiprep Spin columns can be re-used five times and are available as complete kits including buffers or as bulk packs
They are an attractive alternative to lengthy chromatographic methods such as FPLC
For processing larger sample volumes than 20 ml, e
g
from diluted cell culture supernatants, the Vivapure maxiprep Spin columns can additionally be used with a sealing cap and pump collar for peristaltic pumps
For smaller volumes, the Vivapure Miniprep kit is also availablity
Spin column based kit Devices are ready to use Rapid purification with high yields Parallel processing Cost effective through re-use option Starter kits available Processing of larger samples with peristaltic pump