Sartorius Vivapure™ maxiprep MC spin columns

Description

• Vivapure maxiprep MC spin columns are centrifugal devices for rapid purification of His-tagged recombinant proteins from cell lysates by metal chelate affinity chromatography
• The pre-packed spin columns incorporate Ni2+-IDA agarose resin plugs as the chromatographic matrix
• The rapid 1-2-3 bind-wash-elute protocol especially lends itself to screening applications, where many samples are processed in parallel
• Vivapure maxiprep MC spin columns can conveniently be used in a centrifuge with swing-out rotor for standard 50 ml tubes
• Binding capacity is about 10 mg His-tagged protein per unit
• The low hold-up volume ensures high solute recovery with minimal loss by non-specific adsorption
• Vivapure maxiprep MC spin columns can be re-used twice and are available as complete kits including buffers or as bulk packs.They are an attractive alternative to lengthy chromatographic methods such as FPLC
• For processing larger sample volumes than 20 ml, e
• g
• from diluted cell culture supernatants, the Vivapure maxiprep spin columns can additionally be used with a sealing cap and pump collar for peristaltic pumps
• Spin column based kit Devices are ready to use Rapid purification with high yields Parallel processing Cost effective through re-use option Starter kits available Processing of larger samples with peristaltic pump