Thermo Scientific™ CaptureSelect™ Human Albumin Affinity Matrix

Description

• CaptureSelect Human Albumin Affinity Matrix has been specifically designed for the purification of human albumin and albumin fusion proteins
• Features of this affinity matrix include: Highly selective for human albumin Unique elution characteristics allowing for gentle elution of albumin fusion proteins Excellent scalability Non-animal-derived The high selectivity and yields obtained using CaptureSelect Human Albumin Affinity Matrix enable a robust and efficient purification process with excellent purity obtained in one step
• The mild elution conditions make the resin suitable for the purification of albumin fusion proteins, especially those that are pH sensitive
• Free of animal components CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology
• The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free)
• Main Characteristics: Matrix : agarose-based, aldehyde-activated Average particle size : 65 ± 10 μm Ligand : CaptureSelect HSA affinity ligand Ligand coupling method : aldehyde coupling via NH 2 residues of the ligand Binding capacity : ∼15 mg albumin/mL resin Elution conditions (neutral pH) : 20 mM Tris, pH 7.4 with 2 M MgCl 2 or with 1 M NaCl + 50%(v/v) propylene glycol or with 1 M NaCl + 0.5 M L-arginine Elution conditions (acidic pH) : 0.1 M glycine, pH 3.0 Flow characteristics : 50–200 cm/h (up to 2 bar) Formulation buffer : 20%(v/v) ethanol