Thermo Scientific™ CaptureSelect™ IgE Affinity Matrix

Description

• CaptureSelect IgE Affinity Matrix has been designed specifically for the purification of human IgE (immunoglobuline E) from recombinant and plasma sources
• Features of this affinity matrix include: Purification of human IgE, binding different recombinant IgE forms Efficient removal of host cell proteins (HCP) and IgE aggregates Excellent scalability Non–animal-derived Free of animal components CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology
• The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free)
• Main characteristics Matrix: agarose-based, epoxide-activated Average particle size: 65 ± 10 μm Ligand: CaptureSelect IgE affinity ligand Ligand coupling method: aldehyde coupling Binding capacity: >5 mg/mL resin Elution conditions: 50 mM sodium citrate, 150 mM NaCl, pH 3.5 Flow characteristics : 50–200 cm/h (up to 2 bar) Formulation buffer: 20% (v/v) ethanol