Description

• A highly potent and selective oxysterol EBI2 (GPR183) agonist (K d ) = 450 pM in a saturation binding assay, and EC 50 = 140 pM over EC 50 = 2.1nM for its enantiomer, 7β,25-OHC, in a GTP-γS binding assay)
• Dose-dependently suppresses forskolin-induced cAMP accumulation in an EBI2-expressing SK-N-MC/CRE-β-galactosidase cell line (IC 50 = 2nM), but not in control cells
• Stimulates migration of LPS-activated spleen B-cells and anti-CD3/CD28-activated CD4 + T-cells in a dose-dependent manner
• In addition, pharmacological inhibition of its biosynthesis in vivo by Clotrimazole, a CYP7B1inhibitor, promotes the migration of adoptively transferred pre-activated B cells to the T/B boundary, mimicking the phenotype of pre-activated B cells in EBI2-deficient mice.