Thermo Scientific™ POROS™ CaptureSelect™ FibXL Affinity Matrix

Description

• POROS CaptureSelect FibXL Affinity Matrix has specifically been designed for the purification of human fibrinogen from recombinant or plasma source
• Features of this affinity matrix include: Fibrinogen purification from human plasma and recombinant sources Efficient purification of fibrinogen with high yields and retained activity Excellent scalability Non-animal derived High flow rates POROS CaptureSelect FibXL Affinity Matrix is an affinity matrix that binds to fibrinogen with high selectivity and yield, enabling a robust, fast, and efficient purification process with excellent purity obtained in one step
• Free of animal components CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology
• The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free)
• Main characteristics Matrix: cross-linked poly(styrene-divinylbenzene) Average particle size: 50 μm Ligand: POROS CaptureSelect FibXL affinity ligand Ligand coupling method: epoxide Binding capacity: approximately 15 g/L resin (determined with plasma derived fibrinogen) Elution conditions: acidic elution with 0.1 M glycine pH 3.0; neutral pH elution with 20 mM Tris, 1.0 M MgCl 2 and 40% (v/v) propyleneglycol Mechanical resistance: 100 bar (1450 psi, 10 MPa) Formulation buffer: 20 % (v/v) ethanol