Thermo Scientific™ CaptureSelect™ C-tagXL Affinity Matrix

Description

• CaptureSelect C-tagXL Affinity Matrix combines a unique selectivity for a small 4-amino acid peptide tag (E-P-E-A, glutamic acid-proline-glutamic acid-alanine) with the benefits of a robust and high quality affinity matrix
• It purifies C-terminal tagged proteins with high affinity and selectivity, even in the presence of urea and guanidine HCl, from complex mixtures like cell culture harvests and periplasmatic fractions in a one-step process
• Mild elution conditions at neutral pH can be applied using magnesium chloride or propylene glycol, which ensures high activity recoveries of pH-sensitive target proteins
• C-tagXL Affinity Matrix recognizes the E-P-E-A tag sequence when fused either directly to the C-terminus of a protein or through a linker between the C-terminus and the E-P-E-A tag
• Features of C-tagXL Affinity Matrix include: Mild elution, making it suitable for pH-sensitive proteins Binding of the tagged proteins under denaturing conditions (like dissolved inclusion bodies) Excellent scalability Non-animal-derived CaptureSelect C-tagXL Affinity Matrix purifies C-terminal tagged E-P-E-A proteins directly from complex source materials in a single step with high purity and yield
• Free of animal components CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology
• The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free)
• Main characteristics: Matrix: agarose-based, epoxide-activated Average particle size: 65 ± 10 μm Ligand: CaptureSelect C-tagXL affinity ligand Ligand coupling method: epoxide coupling of the ligand Binding capacity: 400 nmol/mL resin depending on flow rate, column height, and contact time Elution conditions: Acidic: 20 mM citric acid or acetic acid, pH 3–4, or 0.1 M glycine pH 3.0; Neutral: 20 mM Tris, 2.0 M MgCl 2 pH 7; 20 mM Tris, 1.0 M NaCl, 50% (v/v) propylene glycol; 20 mM Tris 2 mM S-E-P-E-A
• Flow characteristics: 50–200 cm/h (up to 2 bar) Formulation buffer: 20%(v/v) ethanol