Thermo Scientific™ CaptureSelect™ Factor X Affinity Matrix

Description

• CaptureSelect Factor X Affinity Matrix was specifically designed for the purification of human Factor X from plasma and recombinant sources
• Features of the CaptureSelect Factor X Affinity Matrix include: Highly selective for human Factor X with no affinity for other human plasma proteins Binds both Factor X and Factor Xa in the presence of calcium Enables efficient purification of Factor X with high yields and retained activity Mild elution at neutral pH using EDTA Offers excellent scalability Non-animal-derived Coagulation Factor X is a vitamin K-dependent, liver-produced serine protease that serves as the first enzyme in the coagulation cascade to form fibrin
• It is a two-chain glycoprotein with a molecular weight of approximately 59 kDa
• Factor X normally circulates in the plasma as an inactive molecule with activation of Factor X involved in both the intrinsic and extrinsic coagulation pathways
• Free of animal components CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology
• The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free)
• Main characteristics Matrix: agarose-based Average particle size: 65 ± 10 μm Ligand: CaptureSelect Factor X affinity ligand Ligand coupling method: epoxide Binding capacity: >10 mg/mL resin Binding conditions: 50 mM Tris, 150 mM NaCl, 5 mM CaCl 2 , pH 7 Elution conditions: 50 mM Tris, 150 mM NaCl, 5 mM EDTA, pH 7 Flow characteristics: 50–200 cm/h (up to 2 bar) Formulation buffer: 20% (v/v) ethanol