3667108

Cytiva Con A Sepharose™ 4B Medium

An affinity chromatography media for capture of glycosylated biomolecules including glycoproteins and glycolipids

Manufacturer: Fischer Scientific

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Binding Capacity

30mg/mL

Matrix

4% Agarose

pH

4 to 9

For Use With (Application)

For separation and purification of Glycoproteins, Polysaccharides and Glycolipids, isolates cell surface Glycoproteins from detergent-solubilized membranes

Ligand Concentration

13mg Con A/mL Drained Medium

Particle Size

90 μm

Content And Storage

2°C to 8°C

Ligand

Concanavalin A

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Description

  • Highly suited for isolation of cell surface glycoproteins from detergent-solubilized membranes
  • Con A coupled to Sepharose, in a 4% agarose matrix via cyanogen bromide activation Binds molecules that contain α-D-mannose, α-D-glucose, and sterically related residues with available C-3, C-4, or C-5 hydroxyl groups Group-specific adsorbent for molecules containing sugars Concentration of immobilized Con A, approx
  • 13mg/mL medium; binding capacity for porcine thyroglobulin, approx
  • 30mg/mL medium Stable in the pH range 4 to 9, and in the presence of all commonly used buffers Average particle size: 90μm Delivered in 0.1M acetate buffer, pH 6.0, containing 1M NaCl, 1mM CaCl 2 , 1mM MgCl 2 , 1mM MnCl 2 , and 20% ethanol

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3667108

An affinity chromatography media fo...


Binding Capacity:
30mg/mL

Matrix:
4% Agarose

pH:
4 to 9

For Use With (Application):
For separation and purification of Glycoproteins, Polysaccharides and Glycolipids, isolates cell surface Glycoproteins from detergent-solubilized membranes

Ligand Concentration:
13mg Con A/mL Drained Medium

Particle Size:
90 μm

Content And Storage:
2°C to 8°C

Ligand:
Concanavalin A

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Matrix:
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pH:
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For Use With (Application):
For immunoglobin purification and fractionation and is suitable for immunoprecipitation procedures

Ligand Concentration:
3mg Protein A/mL Medium

Particle Size:
90 μm

Content And Storage:
2°C to 8°C

Ligand:
Native Protein A

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Matrix:
4% Cross Linked Agarose

pH:
3 to 9

For Use With (Application):
Used in a range of diagnostic and research applications

Ligand Concentration:
2mg Protein G/mL Medium

Particle Size:
90 μm

Content And Storage:
2°C to 8°C

Ligand:
Recombinant Protein G lacking albumin binding region

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Matrix:
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pH:
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For Use With (Application):
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Ligand Concentration:
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Particle Size:
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Content And Storage:
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Ligand:
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