3676401
Cytiva HiTrap™ Chelating HP Columns
Optimize histidine-tagged protein purifications when nickel ions are not the best choice. Cytiva Life Sciences™ HiTrap™ Chelating HP Columns is prepacked with Chelating Sepharose™ High Performance.
Manufacturer: Fischer Scientific
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Buffer
6M Guanidine Hydrochloride, 8M Urea
Height (English)
0.98 in.
Ligand Type
Iminodiacetic Acid
pH Range
3 to 13
Support Type
6% Highly Cross-linked Agarose
Column Type
Affinity Column
Product Type
HiTrap chelating HP column
Height (Metric)
25mm
Max. Pressure
3 bar
Particle Size
34μm
Content And Storage
4° to 30°C
For Use With (Application)
IMAC and subsequent purification of Polyhistidine tagged Proteins
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Description
- Uses different kinds of metal ions Binding capacity ∽12mg histidine-tagged protein/mL medium Metal ion capacity ∽23μmol Cu 2 + /mL medium Prepacked with Chelating Sepharose High Performance media Iminoacetic acid coupled to a 6% agarose matrix by ether linkage
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Buffer: 6M Guanidine Hydrochloride, 8M Urea
Height (English): 0.98 in.
Ligand Type: Iminodiacetic Acid
pH Range: 3 to 13
Support Type: 6% Highly Cross-linked Agarose
Column Type: Affinity Column
Product Type: HiTrap chelating HP column
Height (Metric): 25mm
Max. Pressure: 3 bar
Particle Size: 34μm
Content And Storage: 4° to 30°C
For Use With (Application): IMAC and subsequent purification of Polyhistidine tagged Proteins
Buffer:
6M Guanidine Hydrochloride, 8M Urea
Height (English):
0.98 in.
Ligand Type:
Iminodiacetic Acid
pH Range:
3 to 13
Support Type:
6% Highly Cross-linked Agarose
Column Type:
Affinity Column
Product Type:
HiTrap chelating HP column
Height (Metric):
25mm
Max. Pressure:
3 bar
Particle Size:
34μm
Content And Storage:
4° to 30°C
For Use With (Application):
IMAC and subsequent purification of Polyhistidine tagged Proteins
Buffer: __
Height (English): 0.98 in.
Ligand Type: __
pH Range: 4 to 12
Support Type: 6% Highly Cross-linked Agarose
Column Type: __
Product Type: Blue HP Columns
Height (Metric): 2.5cm
Max. Pressure: 42psi
Particle Size: 34 μm
Content And Storage: 4° to 30°C
For Use With (Application): Purifying wide range of biomolecules, including albumin, interferon, a broad range of nucleotide-dependent Enzymes, alpha2-macroglobulin, coagulation facto rs and related Proteins
Buffer:
__
Height (English):
0.98 in.
Ligand Type:
__
pH Range:
4 to 12
Support Type:
6% Highly Cross-linked Agarose
Column Type:
__
Product Type:
Blue HP Columns
Height (Metric):
2.5cm
Max. Pressure:
42psi
Particle Size:
34 μm
Content And Storage:
4° to 30°C
For Use With (Application):
Purifying wide range of biomolecules, including albumin, interferon, a broad range of nucleotide-dependent Enzymes, alpha2-macroglobulin, coagulation facto rs and related Proteins
Buffer: __
Height (English): __
Ligand Type: Glutathione
pH Range: 3 to 12
Support Type: 4% Highly Cross-linked Agarose
Column Type: __
Product Type: __
Height (Metric): __
Max. Pressure: __
Particle Size: 34 μm
Content And Storage: 4°C to 30°C, 20% Ethanol
For Use With (Application): Glutathione S-transferase (GST) tagged proteins produced using the pGEX series of expression vectors, Other glutathione S-transferases and glutathione-dependent proteins
Buffer:
__
Height (English):
__
Ligand Type:
Glutathione
pH Range:
3 to 12
Support Type:
4% Highly Cross-linked Agarose
Column Type:
__
Product Type:
__
Height (Metric):
__
Max. Pressure:
__
Particle Size:
34 μm
Content And Storage:
4°C to 30°C, 20% Ethanol
For Use With (Application):
Glutathione S-transferase (GST) tagged proteins produced using the pGEX series of expression vectors, Other glutathione S-transferases and glutathione-dependent proteins
Buffer: __
Height (English): __
Ligand Type: __
pH Range: __
Support Type: __
Column Type: Column Accesory
Product Type: __
Height (Metric): __
Max. Pressure: __
Particle Size: __
Content And Storage: __
For Use With (Application): __
Buffer:
__
Height (English):
__
Ligand Type:
__
pH Range:
__
Support Type:
__
Column Type:
Column Accesory
Product Type:
__
Height (Metric):
__
Max. Pressure:
__
Particle Size:
__
Content And Storage:
__
For Use With (Application):
__