45-002-072
Cytiva HiPrep™ IMAC FF 16/10 Column
Manufacturer: Cytiva
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 45-002-072-Each-of-1 | In Stock | ₹ 62,287.68 |
45-002-072 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 62,287.68
GST (18%): ₹ 11,211.782
Total Price: ₹ 73,499.462
Buffer
0.01M HCl, 1M NaOH, 70% Acetic Acid, 2% SDS, 30% 2-propanol
Bed Volume
20mL
Diameter (Metric) Inner
16mm
Height (English)
3.93 in.
Flow Rate
<10mL/min.
Max. Pressure
1.5 bar
Particle Size
45 to 165μm
Content And Storage
4° to 30°C
For Use With (Application)
IMAC and subsequent purification of Polyhistidine tagged Proteins
Product Type
HiTrap IMAC Sepharose 6 FF column
Diameter (English) Inner
0.62 in.
Quantity
1 x 20mL
Height (Metric)
100mm
Material
Polypropylene
pH Range
3 to 12
Support Type
6% Cross-linked Agarose
Column Type
Ion Exchange Column
Related Products
Description
- IMAC Sepharose™ 6 Fast Flow is prepacked in 20mL HiPrep columns for greater scale up
- The medium consists of 90μm beads of highly crosslinked agarose, to which a chelating group has been covalently coupled
- This group may be charged with suitable metal ions, specific for your target protein.The bead size enables high flow properties; low metal ion leakage retains activity of the purified protein
- Specifications: IMAC Sepharose 6 Fast Flow Matrix: highly crosslinked agarose, 6% Average particle size: 90μm Binding capacity (protein/ion dependent): approx
- 40mg histidine-tagged protein/mL medium when charged with Ni 2+ Compatibility: stable in all commonly used buffers Chemical stability (uncharged medium) one week, 40°C: 0.01 M HCl, 0.1 M NaOH; 12 hr.: 1M NaOH, 70% acetic acid; 1 hr
- tested 2% SDS; 30 min
- tested 30% 2-propanol Stability (pH, w/ uncharged medium w/ metal ion): 3 to 12 (long-term), 2 to 14 (short-term) Storage/Temperature: 20% ethanol; 4° to 30°C HiPrep Prepacked Columns Volume: 20mL Dimensions: 1.6 x 10cm Max
- pressure during operation: 1.5bar (0.15MPa, 22psi) Hardware pressure limit: 5bar (0.5MPa, 73psi) Recommend flowrate (H 2 O at room temp.): 2 to 10mL/min.
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Buffer: 0.01M HCl, 1M NaOH, 70% Acetic Acid, 2% SDS, 30% 2-propanol
Bed Volume: 20mL
Diameter (Metric) Inner: 16mm
Height (English): 3.93 in.
Flow Rate: <10mL/min.
Max. Pressure: 1.5 bar
Particle Size: 45 to 165μm
Content And Storage: 4° to 30°C
For Use With (Application): IMAC and subsequent purification of Polyhistidine tagged Proteins
Product Type: HiTrap IMAC Sepharose 6 FF column
Diameter (English) Inner: 0.62 in.
Quantity: 1 x 20mL
Height (Metric): 100mm
Material: Polypropylene
pH Range: 3 to 12
Support Type: 6% Cross-linked Agarose
Column Type: Ion Exchange Column
Buffer:
0.01M HCl, 1M NaOH, 70% Acetic Acid, 2% SDS, 30% 2-propanol
Bed Volume:
20mL
Diameter (Metric) Inner:
16mm
Height (English):
3.93 in.
Flow Rate:
<10mL/min.
Max. Pressure:
1.5 bar
Particle Size:
45 to 165μm
Content And Storage:
4° to 30°C
For Use With (Application):
IMAC and subsequent purification of Polyhistidine tagged Proteins
Product Type:
HiTrap IMAC Sepharose 6 FF column
Diameter (English) Inner:
0.62 in.
Quantity:
1 x 20mL
Height (Metric):
100mm
Material:
Polypropylene
pH Range:
3 to 12
Support Type:
6% Cross-linked Agarose
Column Type:
Ion Exchange Column
Buffer: 1M Acetate, pH 6, 0.1M NaOH, 70% Ethanol or 6M Guanidine Hydrochloride
Bed Volume: 20mL
Diameter (Metric) Inner: 16mm
Height (English): 3.9 in.
Flow Rate: <10mL/min.
Max. Pressure: 1.5 bar
Particle Size: 90μm
Content And Storage: 4° to 30°C
For Use With (Application): Purification of Glutathione S-transferases and Glutathione-dependent Proteins
Product Type: GSTPrep Glutathione Sepharose 4 FF column
Diameter (English) Inner: 0.62 in.
Quantity: 1 x 20mL
Height (Metric): 100mm
Material: __
pH Range: 3 to 12
Support Type: 4% Highly Cross-linked Agarose
Column Type: Ion Exchange Column
Buffer:
1M Acetate, pH 6, 0.1M NaOH, 70% Ethanol or 6M Guanidine Hydrochloride
Bed Volume:
20mL
Diameter (Metric) Inner:
16mm
Height (English):
3.9 in.
Flow Rate:
<10mL/min.
Max. Pressure:
1.5 bar
Particle Size:
90μm
Content And Storage:
4° to 30°C
For Use With (Application):
Purification of Glutathione S-transferases and Glutathione-dependent Proteins
Product Type:
GSTPrep Glutathione Sepharose 4 FF column
Diameter (English) Inner:
0.62 in.
Quantity:
1 x 20mL
Height (Metric):
100mm
Material:
__
pH Range:
3 to 12
Support Type:
4% Highly Cross-linked Agarose
Column Type:
Ion Exchange Column
Buffer: 1M NaOH, 30% Isopropanol, 70% Ethanol, 6M Guanidine Hydrochloride, 30% Aceto nitrile, 1mM HCl
Bed Volume: 20mL
Diameter (Metric) Inner: 16mm
Height (English): 3.93 in.
Flow Rate: >300 cm/hr.
Max. Pressure: 5 bar
Particle Size: 45 to 165μm
Content And Storage: 4° to 30°C
For Use With (Application): Provide fast, preparative separation of Proteins and other biomolecules by hydrophobic interaction chromato graphy (HIC), initial and intermediate purification, removing bulk impurities
Product Type: Octyl Sepharose
Diameter (English) Inner: 0.62 in.
Quantity: 1 x 20 mL
Height (Metric): 100mm
Material: Polypropylene
pH Range: 3 to 13
Support Type: 4% Cross-linked Agarose
Column Type: Ion Exchange Column
Buffer:
1M NaOH, 30% Isopropanol, 70% Ethanol, 6M Guanidine Hydrochloride, 30% Aceto nitrile, 1mM HCl
Bed Volume:
20mL
Diameter (Metric) Inner:
16mm
Height (English):
3.93 in.
Flow Rate:
>300 cm/hr.
Max. Pressure:
5 bar
Particle Size:
45 to 165μm
Content And Storage:
4° to 30°C
For Use With (Application):
Provide fast, preparative separation of Proteins and other biomolecules by hydrophobic interaction chromato graphy (HIC), initial and intermediate purification, removing bulk impurities
Product Type:
Octyl Sepharose
Diameter (English) Inner:
0.62 in.
Quantity:
1 x 20 mL
Height (Metric):
100mm
Material:
Polypropylene
pH Range:
3 to 13
Support Type:
4% Cross-linked Agarose
Column Type:
Ion Exchange Column
Buffer: 1M NaOH, 30% Isopropanol, 70% Ethanol, 6M Guanidine Hydrochloride, 30% Aceto nitrile, 1mM HCl
Bed Volume: 20mL
Diameter (Metric) Inner: 16mm
Height (English): 3.93 in.
Flow Rate: >300 cm/hr.
Max. Pressure: 5 bar
Particle Size: 45 to 165μm
Content And Storage: 4° to 30°C
For Use With (Application): Provide fast, preparative separation of Proteins and other biomolecules by hydrophobic interaction chromato graphy (HIC), initial and intermediate purification, removing bulk impurities
Product Type: Butyl Sepharose
Diameter (English) Inner: 0.62 in.
Quantity: 1 x 20 mL
Height (Metric): 100mm
Material: Polypropylene
pH Range: 3 to 13
Support Type: 4% Cross-linked Agarose
Column Type: Ion Exchange Column
Buffer:
1M NaOH, 30% Isopropanol, 70% Ethanol, 6M Guanidine Hydrochloride, 30% Aceto nitrile, 1mM HCl
Bed Volume:
20mL
Diameter (Metric) Inner:
16mm
Height (English):
3.93 in.
Flow Rate:
>300 cm/hr.
Max. Pressure:
5 bar
Particle Size:
45 to 165μm
Content And Storage:
4° to 30°C
For Use With (Application):
Provide fast, preparative separation of Proteins and other biomolecules by hydrophobic interaction chromato graphy (HIC), initial and intermediate purification, removing bulk impurities
Product Type:
Butyl Sepharose
Diameter (English) Inner:
0.62 in.
Quantity:
1 x 20 mL
Height (Metric):
100mm
Material:
Polypropylene
pH Range:
3 to 13
Support Type:
4% Cross-linked Agarose
Column Type:
Ion Exchange Column