Cytiva Capto™ Core 700

Description

• The novel core bead technology and multimodal, octylamine ligand give Capto Core 700 dual functionality, size exclusion, and binding chromatography in one chromatography medium (resin)
• For intermediate purification and polishing of viruses and other large biomolecules (Mr > 700 000) in flowthrough mode
• Novel core bead technology allows efficient capture of contaminants while target molecules are collected in the flowthrough
• Significantly improved productivity and higher flow rates compared with gel filtration methods
• Straightforward optimization due to flowthrough chromatography and robust performance
• Convenient small-scale purification, process development, and scale-up using prepacked HiTrap and HiScreen columns
• Capto Core 700 is composed of a ligand-activated core and inactive shell
• The inactive shell excludes large molecules (cut off ∼ Mr 700000 [700 kDa]) from entering the core through the pores of the shell
• These larger molecules are collected in the column flowthrough while smaller impurities bind to the internalized ligands
• The core of each bead is functionalized with ligands that are both hydrophobic and positively charged, resulting in a highly efficient multimodal binding of various contaminants small enough to enter the core
• The multimodal ligands ensure strong binding with most impurities over a wide range of pH and salt concentrations
• Bound impurities are removed from the beads by cleaning-in-place (CIP) procedures using NaOH and in most cases, a solvent.