4513005
Cytiva HisTrap™ excel Prepacked Columns
For capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants. Cytiva Lifescience™ HisTrap™ excel Prepacked Columns are designed for fast and convenient purifications.
Manufacturer: Fischer Scientific
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Description
HisTrap excel columns are prepacked with Ni Sepharose excel affinity resins for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants by immobilized metal ion affinity chromatography (IMAC).
pH Range
2 to 12
Content And Storage
4°C to 30°C
Type
Pre-packed Column
Material
Polypropylene
Support Type
6% Highly Cross-linked Agarose
Max. Operating Pressure
5 bar (0.5 MPa, 70 psi)
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Description
- The design of the columns in combination with the specific properties of the medium enables fast and convenient purifications
- Ready-to use prepacked with Ni Sepharose excel Special type of filter in top and bottom of the columns to load large volumes of cell-free, un clarified samples directly on the columns helps prevent degradation and loss of sensitive target proteins Made of biocompatible polypropylene and cannot be opened or refilled Delivered with stopper on inlet and snap-off end on outlet Operated either with peristaltic pump or with chromatography systems such as KTA systems Binding Capacity/Column: At least 10 mg histidine-tagged protein/ml sedimented medium Media Average particle size: 90μm Matrix: Highly cross-linked agarose, 6% pH Stability working range: 2 to 12 pH Stability cleaning range: 2 to 14 Flow velocity: 150 cm/h - 600 cm/h 4 Column: Column I.D.: 7 mm HisTrap excel 1mL column Bed Dimensions: 7 × 25mm Flow rate: 1 to 4mL/min
- HisTrap excel 5mL column Bed Dimensions: 16 × 25mm Flow rate: 5 to 20mL/min.
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Description: HisTrap excel columns are prepacked with Ni Sepharose excel affinity resins for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants by immobilized metal ion affinity chromatography (IMAC).
pH Range: 2 to 12
Content And Storage: 4°C to 30°C
Type: Pre-packed Column
Material: Polypropylene
Support Type: 6% Highly Cross-linked Agarose
Max. Operating Pressure: 5 bar (0.5 MPa, 70 psi)
Description:
HisTrap excel columns are prepacked with Ni Sepharose excel affinity resins for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants by immobilized metal ion affinity chromatography (IMAC).
pH Range:
2 to 12
Content And Storage:
4°C to 30°C
Type:
Pre-packed Column
Material:
Polypropylene
Support Type:
6% Highly Cross-linked Agarose
Max. Operating Pressure:
5 bar (0.5 MPa, 70 psi)
Description: __
pH Range: 2 to 12
Content And Storage: 4°C to 30°C
Type: Chromatography Media
Material: __
Support Type: Highly cross-linked spherical Agarose including Magnetite
Max. Operating Pressure: __
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pH Range:
2 to 12
Content And Storage:
4°C to 30°C
Type:
Chromatography Media
Material:
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Support Type:
Highly cross-linked spherical Agarose including Magnetite
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