4513584
Cytiva Ni Sepharose™ 6 Fast Flow
Combines the advantages of Ni2 for purification of histidine tagged proteins, with the well industrially established properties of the Sepharose Fast Flow platform. Cytiva Lifescience™ Ni Sepharose™ 6 Fast Flow is part of a range of IMAC chromatography media for capture and intermediate purification.
Manufacturer: Fischer Scientific
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Buffer
0.1M HCl, 0.1M NaOH, 8M Urea
Height (English)
9.84 in.
Flow Rate
600cm/hr.
pH Range
3 to 12
Support Type
6% Cross-linked Agarose
Matrix
Ni-Sepharose
Product Type
Ni Sepharose 6 FF chromatography media
Height (Metric)
250mm
Max. Pressure
1 bar
Particle Size
90μm
Content And Storage
4° to 30°C
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Description
- Ni Sepharose 6 Fast Flow is a member of the Cytiva range of IMAC chromatography media for capture and intermediate purification
- Designed for scaling up purification of histidine-tagged proteins
- High protein binding capacity and minimal leakage of Ni2+ ions
- As a BioProcess medium it fulfills industrial demands for security of supply, robust performance and regulatory support
- Available in convenient prepacked HisPrep and HisTrap column formats
- Composed of cross-linked 6% agarose beads modified with a novel chelating ligand immobilized to the base matrix and pre-charged with Ni2+ ions for purification of histidine tagged proteins
- The coupling chemistry and the novel ligand is designed to give minimal ligand and Ni2+ leakage while providing high capacity
- Available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development
- As member of the BioProcess media range, Ni Sepharose 6 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.
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Buffer: 0.1M HCl, 0.1M NaOH, 8M Urea
Height (English): 9.84 in.
Flow Rate: 600cm/hr.
pH Range: 3 to 12
Support Type: 6% Cross-linked Agarose
Matrix: Ni-Sepharose
Product Type: Ni Sepharose 6 FF chromatography media
Height (Metric): 250mm
Max. Pressure: 1 bar
Particle Size: 90μm
Content And Storage: 4° to 30°C
Buffer:
0.1M HCl, 0.1M NaOH, 8M Urea
Height (English):
9.84 in.
Flow Rate:
600cm/hr.
pH Range:
3 to 12
Support Type:
6% Cross-linked Agarose
Matrix:
Ni-Sepharose
Product Type:
Ni Sepharose 6 FF chromatography media
Height (Metric):
250mm
Max. Pressure:
1 bar
Particle Size:
90μm
Content And Storage:
4° to 30°C
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