MilliporeSigma™ Calbiochem™ Cell Proliferation Inducer, WS3

Description

• A cell-permeable, diarylurea analog that can reversibly induce cell proliferation of primary retinal pigment epithelial (RPE) cells from rodents and from fetal and adult human donors (∽ 25nM)
• Its action suggested to be mediated through Erb3 binding protein 1 (Ebp1)
• Non-proliferating RPE cells at passage 6 appear to respond well to WS3 treatment, whereas actively proliferating cells in passage 1 to 4 do not exhibit any increase in proliferation
• These cells can differentiate into functional monolayer following WS3 withdrawal
• However, WS3-expanded adult human RPE cells differentiate less effectively when compared to stem cell-derived RPE cells
• The expanded RPE cells are shown to preserve vision when transplanted into rodent models of retinal degeneration
• Also shown to induce proliferation of mouse R7T1 β cells (EC 50 = 74nM)
• Not reported to induce any genetic changes or tumorigenic events.